{"created":"2023-07-27T06:51:14.835996+00:00","id":44597,"links":{},"metadata":{"_buckets":{"deposit":"2c423c19-9bf8-4fb6-a6c0-1465b129a880"},"_deposit":{"created_by":18,"id":"44597","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"44597"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00044597","sets":["2812:2813:2817"]},"author_link":["76684","76685"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-06-01","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"6p.","bibliographicVolumeNumber":"2014-04-01 - 2017-03-31","bibliographic_titles":[{"bibliographic_title":"平成28(2016)年度 科学研究費補助金 若手研究(B) 研究成果報告書"},{"bibliographic_title":"2016 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ボツリヌス菌が産生するボツリヌス神経毒素(BoNT)は、重篤な食中毒である食餌性ボツリヌス症を引き起こす。BoNTは無毒タンパク質(NAPs）と複合体を形成し、NAPの機能によって宿主腸管から効率よく吸収される。本複合体はpH 7.0以上の緩衝液中でBoNTとNAPsに解離することから、弱アルカリ性である宿主腸管でも解離すると考えられてきた。\n本研究では、宿主腸管に存在する宿主由来因子が、弱アルカリ性環境下で本複合体の解離を阻害することを明らかにした。このことは、宿主腸管内でも本毒素複合体が解離しないことを示唆しており、本研究によってボツリヌス食中毒の発症機構における重要な知見が得られた。","subitem_description_type":"Abstract"},{"subitem_description":"Clostridium botulinum neurotoxin (BoNT) exists as progenitor toxin complexes (PTCs) with nontoxic components such as hemagglutinin (HA) and non-toxic non-HA (NTNHA). In PTC molecules, BoNT associates with NTNHA noncovalently. NTNHA protects BoNT from digestion and HA facilitates BoNT absorption in host digestive tract.\nBoNT dissociates from NTNHA in weak alkaline buffer (pH > 7.0) in vitro, so that BoNT is assumed to be present as a released form in host intestine. Meanwhile, it is reported that the BoNT does not dissociate in rat intestinal fluid (pH 7.0) by sucrose gradient ultracentrifugation analysis. However, further studies were not performed.\nIn this research, we found that the mouse intestinal fluid fraction, which contains heat-resistant small molecular weight compounds, inhibits the dissociation between BoNT and NAPs in alkaline conditions. This result suggests that BoNT does not dissociate from NAP in host intestinal tract.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:26860287, 研究期間(年度):2014-04-01 - 2017-03-3","subitem_description_type":"Other"},{"subitem_description":"出典：「宿主消化管内におけるボツリヌス毒素の動態解明」研究成果報告書　課題番号26860287\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26860287/26860287seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00050938","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=20648810"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=20648810","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26860287/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26860287/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26860287/26860287seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26860287/26860287seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-06-04"}],"displaytype":"detail","filename":"ME-PR-YUTANI-M-kaken 2017-4p.pdf","filesize":[{"value":"295.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-YUTANI-M-kaken 2017-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/44597/files/ME-PR-YUTANI-M-kaken 2017-4p.pdf"},"version_id":"1c71a68e-d844-4964-92e4-67fe2c7e129f"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"宿主消化管内におけるボツリヌス毒素の動態解明","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"宿主消化管内におけるボツリヌス毒素の動態解明"},{"subitem_title":"The study of molecular assembly of botulinum neurotoxin complex in host intestine","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2817"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-06-04"},"publish_date":"2018-06-04","publish_status":"0","recid":"44597","relation_version_is_last":true,"title":["宿主消化管内におけるボツリヌス毒素の動態解明"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T11:35:59.618086+00:00"}