{"created":"2023-07-27T06:51:14.970905+00:00","id":44600,"links":{},"metadata":{"_buckets":{"deposit":"98918860-0bd8-4654-9db7-939f32f17aab"},"_deposit":{"created_by":18,"id":"44600","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"44600"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00044600","sets":["2812:2813:2817"]},"author_link":["76689","441"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-05-29","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2014-04-01 - 2017-03-31","bibliographic_titles":[{"bibliographic_title":"平成28(2016)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2016 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"【CTCを用いた遺伝子発現解析】小細胞肺癌12例の末梢血単核球分画からRNAを抽出し、RT-PCR法にてCTC由来遺伝子発現の解析を行った。神経内分泌関連および上皮間葉移行関連、血管新生関連の遺伝子発現を検討し、上皮間葉移行関連遺伝子発現を検出することができた。本方法でCTC関連遺伝子発現を検出できることが示唆された。\n【血中DNAを用いた遺伝子変異解析】進行期肺癌剖検例4例を対象に、腫瘍組織由来のDNAと血中DNAを用いて、次世代シークエンサーにて遺伝子変異を解析した。血中DNA中の遺伝子変異は、複数病変に共通して存在し腫瘍組織内のallele frequencyが有意に高かった。","subitem_description_type":"Abstract"},{"subitem_description":"[Gene expression analysis using circulating tumor cells (CTCs)] We extracted RNA from the peripheral blood mononuclear cell fraction of 12 small-cell lung cancer patients and analyzed it for CTC-derived gene expression by the reverse transcriptase-polymerase chain reaction (RT-PCR). We investigated neuroendocrine-related, epithelial-mesenchymal-transition-related, and angiogenesis-related gene expression and were able to identify epithelial-mesenchymal transition-related gene expression. The results suggested that it is possible to identify CTC-related gene expression by this method.\n[Gene mutation analysis using blood DNA] We analyzed tumor tissue derived DNA and blood DNA from 4 autopsy cases of advanced lung cancer by using a next generation sequencer. The same gene mutations as in the blood DNA were present in multiple lesions, and allele frequency in the tumor tissue was significantly higher.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:26430142, 研究期間(年度):2014-04-01 - 2017-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「血中循環がん細胞解析を利用した小細胞肺癌の新規治療標的・バイオマーカーの同定」研究成果報告書　課題番号26430142\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26430142/26430142seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00050941","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=40444202"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40444202","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26430142/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26430142/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26430142/26430142seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26430142/26430142seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-06-04"}],"displaytype":"detail","filename":"ME-PR-KIMURA-H-kaken 2017-4p.pdf","filesize":[{"value":"259.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-KIMURA-H-kaken 2017-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/44600/files/ME-PR-KIMURA-H-kaken 2017-4p.pdf"},"version_id":"efe10415-cd70-46fc-9ff3-c6120765d414"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"血中循環がん細胞解析を利用した小細胞肺癌の新規治療標的・バイオマーカーの同定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"血中循環がん細胞解析を利用した小細胞肺癌の新規治療標的・バイオマーカーの同定"},{"subitem_title":"Identification of Novel Treatment Targets and Biomarkers for Small-Cell Lung Cancer by Circulating Tumor Cell Analysis","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2817"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-06-04"},"publish_date":"2018-06-04","publish_status":"0","recid":"44600","relation_version_is_last":true,"title":["血中循環がん細胞解析を利用した小細胞肺癌の新規治療標的・バイオマーカーの同定"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T11:34:30.224837+00:00"}