{"created":"2023-07-27T06:51:17.453385+00:00","id":44658,"links":{},"metadata":{"_buckets":{"deposit":"dcdd828e-49ce-4f48-9af6-bef8abc0ba2d"},"_deposit":{"created_by":18,"id":"44658","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"44658"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00044658","sets":["2812:2813:2819"]},"author_link":["76772","406"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-05-29","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2013-04-01 - 2015-03-31","bibliographic_titles":[{"bibliographic_title":"平成26(2014)年度 科学研究費補助金 若手研究(B) 研究成果報告書"},{"bibliographic_title":"2014 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ヒト進行胃癌の手術検体から、同一腫瘍内においてHER2発現が陽性および陰性の領域を含む症例を選び出し、そのそれぞれの領域を含む切片からRNAを抽出しマイクロアレイによる遺伝子発現の網羅的解析を行った。その結果、apoptosis関連遺伝子を含む少数の遺伝子に関しては、その両領域間で４倍以上の発現の差が観察された。その値の検証のためにreal-time RT-PCR法を行ったが、RNAの分解のため、再現性のある結果を得ることが困難であった。より再現性の高い結果を得るためにgenomic DNAを用いたSNP arrayを行い、上述の両領域間で互いに増幅の程度が異なるいくつかの遺伝子の候補を得た。","subitem_description_type":"Abstract"},{"subitem_description":"The tumors with intratumoral heterogeneity of HER2 protein were selected from the surgical specimens of HER2 positive advanced gastric cancer. Total RNA were isolated from both HER2 positive and HER2 negative area in a tumor. Whole genome comparative analyses using gene expression arrays of these RNA revealed that the expression levels of some gene including apoptosis associated genes were more than four times as high as those in the other area. The results of these gene expression levels were attempted to be validate by quantitative real-time RT-PCR, but failed to confirm the values of these gene expressions. This might be due to RNA degradation by aging of the tissues and/or formalin fixation. To acquire more reproducible data, Genome-Wide human SNP array analyses were performed on the genomic DNA from the two areas. These analyses revealed the difference in the gene copy number of some genes including a homeobox as were confirmed using fluolecence in situ hybridization.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:25860266, 研究期間(年度):2013-04-01 - 2015-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「胃がんにおけるHER2発現の腫瘍内ヘテロ不均一性に対するDNAメチル化異常の意義」課題番号25860266\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-25860266/25860266seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00050999","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=00515314"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=00515314","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25860266/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25860266/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-25860266/25860266seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-25860266/25860266seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-06-07"}],"displaytype":"detail","filename":"ME-PR-OYAMA-T-kaken 2015-4p.pdf","filesize":[{"value":"304.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-OYAMA-T-kaken 2015-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/44658/files/ME-PR-OYAMA-T-kaken 2015-4p.pdf"},"version_id":"ec62f849-bb62-4086-a3d4-018b279e48b8"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"胃がんにおけるHER2発現の腫瘍内ヘテロ不均一性に対するDNAメチル化異常の意義","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"胃がんにおけるHER2発現の腫瘍内ヘテロ不均一性に対するDNAメチル化異常の意義"},{"subitem_title":"Investigation of the significance of alteration in gene promoter methylations for cause intratumoral heterogeneity of the expression levels of HER2","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2819"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-06-07"},"publish_date":"2018-06-07","publish_status":"0","recid":"44658","relation_version_is_last":true,"title":["胃がんにおけるHER2発現の腫瘍内ヘテロ不均一性に対するDNAメチル化異常の意義"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T10:43:32.966841+00:00"}