{"created":"2023-07-27T06:51:19.749854+00:00","id":44712,"links":{},"metadata":{"_buckets":{"deposit":"e4e93525-d8b3-4f01-8fce-06a74f880bee"},"_deposit":{"created_by":18,"id":"44712","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"44712"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00044712","sets":["2812:2813:2817"]},"author_link":["77011","151"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-06-09","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5p.","bibliographicVolumeNumber":"2014-04-01 - 2017-03-31","bibliographic_titles":[{"bibliographic_title":"平成28(2016)年度 科学研究費補助金 基盤研究(B) 研究成果報告書"},{"bibliographic_title":"2016 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"前立腺癌細胞とヌードマウスを用いて骨転移初期変化の蛍光イメージングを行い、シングルセルレベルでの転移巣初期の変化として、腫瘍が骨に到達して転移巣を形成する過程を継時的に可視化することに成功した。\n骨軟部肉腫における新規治療法として、蛍光ガイド下腫瘍切除術、腫瘍特異的バクテリア（A1-R）治療、GSK-3β阻害薬の有用性について検討した。蛍光ガイド下手術は前立腺癌の骨転移モデルにおいて腫瘍再発を有意に抑制した。A1-Rは軟部肉腫モデルと骨転移モデルのいずれにおいても腫瘍抑制効果を示した。GSK-3β阻害薬は骨肉腫モデルにおいて有意な腫瘍抑制効果を示した。","subitem_description_type":"Abstract"},{"subitem_description":"We demonstrate in vivo fluorescence imaging of bone-marrow micrometastasis of prostate cancer at the cellular level in nude mice using PC-3 human prostate cancer cells labeled with green fluorescent protein (GFP). PC-3-GFP cells were visualized by real-time fluorescence imaging, to traffic and grow in the bone marrow. Formation of bone marrow micrometastasis could be imaged at the single-cell level in live mice, using confocal microscopy.\nWe investigated the efficacies of fluorescence-guided surgery (FGS), genetically-modified auxotrophic strain of Salmonella typhimurium (A1-R), and glycogen synthase kinase-3β (GSK- 3β) inhibitors. FGS significantly reduced residual tumor as well as the recurrence of experimental prostate-cancer bone metastasis. A1-R significantly inhibited tumor growth and metastasis in soft tissue tumor model and bone metastasis model. Treatment with GSK-3β inhibitors attenuated the growth of orthotopic osteosarcoma in mice.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:26293333, 研究期間(年度):2014-04-01 - 2017-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「蛍光イメージングを用いた悪性骨軟部腫瘍の転移機序の解明と新規治療法の開発」研究成果報告書　課題番号26293333\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26293333/26293333seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00051053","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=40227434"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40227434","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26293333/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26293333/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26293333/26293333seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26293333/26293333seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-06-14"}],"displaytype":"detail","filename":"ME-PR-TSUCHIYA-H-kaken 2017-5p.pdf","filesize":[{"value":"272.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-TSUCHIYA-H-kaken 2017-5p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/44712/files/ME-PR-TSUCHIYA-H-kaken 2017-5p.pdf"},"version_id":"80d0b901-0b21-4cda-992c-be6f1f578995"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"蛍光イメージングを用いた悪性骨軟部腫瘍の転移機序の解明と新規治療法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"蛍光イメージングを用いた悪性骨軟部腫瘍の転移機序の解明と新規治療法の開発"},{"subitem_title":"Fluorescence imaging for investigation and treatment of bone and soft tissue tumors","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2817"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-06-14"},"publish_date":"2018-06-14","publish_status":"0","recid":"44712","relation_version_is_last":true,"title":["蛍光イメージングを用いた悪性骨軟部腫瘍の転移機序の解明と新規治療法の開発"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T11:31:52.632430+00:00"}