{"created":"2023-07-27T06:51:19.929727+00:00","id":44716,"links":{},"metadata":{"_buckets":{"deposit":"81fae27f-3c2f-4fae-8eb4-49c76d2e2258"},"_deposit":{"created_by":18,"id":"44716","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"44716"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00044716","sets":["2812:2813:2827"]},"author_link":["77011","151"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-05","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"12p.","bibliographicVolumeNumber":"2004-2006","bibliographic_titles":[{"bibliographic_title":"平成18(2006)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2006 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"カフェイン(以下,CAF)を封入したポリエチレングリコール付加リボゾームを作成した.CAF封入リポゾーム(以下,CAF-L)をラットに静脈内投与した結果,CAFを投与した場合と比較して有意に血中滞留性が優れていることがわかった.ただ,リボゾーム内のCAF封入率が低く,CAFとして充分な量を投与することができず,その臨床効果を評価するにいたらなかった.\nこのリポゾームの腫瘍への集積性を評価するため,蛍光色素を封入したリポゾームを作成した.これは自己消失リポゾームであり,リポゾームから蛍光色素から漏出しない限り発光しないものであった.これを骨肉腫を皮下移植したラットに静脈内投与し,24時間に取り出した腫瘍内に強い発光を認めた.この結果,このリポゾームには腫瘍への有効な集積が期待できるものと判断し,シスプラチン(以下,CDDP)を封入したリポゾームを作成した.このCDDP封入リポゾーム(以下,CDDP-L)の体内動態や抗腫瘍効果,更にカフェインとの併用での抗腫瘍効果について評価した.まず,ラットへCDDPとCDDP-Lを静脈内投与した結果,CDDPに比較しCDDP-Lでは有意に血中滞留性に優れていた.次に,骨肉腫を皮下移植したラットにCDDPとCDDP-Lをそれぞれ投与し,プラチナの腫瘍内濃度を測定し,比較検討した.12時間後ではCDDP群もCDDP-L群も同様のプラチナ濃度であったが,24時間後ではCDDP群でほぼ測定できない値であった一方で,CDDP-L群では12時間後よりも有意に腫瘍内濃度が上昇していた.そして,骨肉腫を皮下移植したラットに対する腫瘍縮小効果を検討した.CDDPとCDDP-Lをそれぞれ1.75mg/kg静脈内投与し,14日後の腫瘍のサイズは両群ともほぼ差は無かった.次に,カフェインとの相乗効果を検討するため,CDDPまたはCDDP-Lを1.75mg/kg静脈内投与後,カフェイン(100mg/kg/day)を3日間投与した群と17日間投与した群を設定し,比較した.4つの群のうち,CDDP-L投与後カフェインを7日間投与した群で,他の3群より有意に腫瘍抑制効果を認めた.以上より,CDDP-Lはそのものの効果としては,CDDPと同等ではあるが,腫瘍内への持続的な抗癌剤の徐放効果を有しており,そのためカフェインの連日投与でより効果的な抗腫瘍効果が得られるものと判断した.","subitem_description_type":"Abstract"},{"subitem_description":"We prepared the liposome with amphipathic polyethylene glycol (PEG) encapsulating caffeine (CAF-L). At first we administered CAF-L to rat via vein, and it was proved that CAF-L had a property of prolonged circulation in rat. But the quantity of caffeine encapsulated in liposome was is not high enough for us to assess the clinical effect of CAF-L.\nTo assess the accumulation of liposome in osteosarcoma, we prepared the liposome encapsulating the fluorescent material (fluoresceine). The character of this liposome is that fluoresceine can emit light only that the liposome is ruptured. We administered this liposome to a rat transplanted solid osteosarcoma subcutaneously. The specimen from the tumor excised in 24 hours after injection via vein of the liposome emitted light enough under a microscope. From this result we concluded the liposome had a property accumulating to a solid osteosarcoma, we prepared the liposome encapsulating cisplatin (CDDP-L) by the same method. We investigated experi mentally in vivo the movement, the antitumor effect and the effect by administration with caffeine. Firstly we administered liquid cisplatin or CDDP-L to rats, and it was become clear that CDDP-L was prolonged circulating. Secondly we administered liquid cisplatin or CDDP-L to the rats subcutaneously transplanted solid osteosarcoma and measured the concentration of platinum in the tumor. The concentration of platinum in the tumor at 12 hours after injection of CDDP-L was almost equal to that after injection of liquid cisplatin, but the concentration at 24 hours after injection of CDDP-L was significantly superior to that after injection of liquid cisplatin. Then we examined the antitumor effect of CDDP-L in vivo. The tumor size at 14 days after venous injection of CDDP-L in dose of 1.75 mg/kg was not different from after injection of liquid cisplatin in the same dose. And we investigated the antitumor effect by administration of CDDP-L with caffeine. We set up the four groups; a group (I) administered one shot liquid cisplatin and the following injected of caffeine in three days, a group (II) one shot liquid cisplatin and the following caffeine in seven days, a group (III) one shot CDDP-L and the following caffeine in three days, a group (VI) one shot CDDP-L and the following caffeine in seven days. In the four group, at the group VI the antitumor effect was superior significantly than the other groups statistically. From this result we concluded that CDDP-L was continuously releasing cisplatin to a solid tumor for a long time and the a long spell of administration of the following caffeine enhanced the antitumor effect of CDDP-L.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:16591480, 研究期間(年度):2004-2006","subitem_description_type":"Other"},{"subitem_description":"出典：「抗癌剤およびカフェイン封入リポゾームを用いた骨肉腫の治療とテーラーメイド化学療法」研究成果報告書　課題番号16591480\n  (KAKEN：科学研究費助成事業データベース（国立情報学研究所）)\n　　　本文データは著者版報告書より作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00051057","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学医薬保健研究域医学系"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=40227434"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40227434","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16591480/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16591480/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16591480/165914802006kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16591480/165914802006kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-06-14"}],"displaytype":"detail","filename":"ME-PR-TSUCHIYA-H-kaken 2007-12p.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-TSUCHIYA-H-kaken 2007-12p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/44716/files/ME-PR-TSUCHIYA-H-kaken 2007-12p.pdf"},"version_id":"d8c1993b-0c65-46eb-92f4-dce0f086c2d5"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"抗癌剤およびカフェイン封入リポゾームを用いた骨肉腫の治療とテーラーメイド化学療法","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"抗癌剤およびカフェイン封入リポゾームを用いた骨肉腫の治療とテーラーメイド化学療法"},{"subitem_title":"Research of the chemotherapy for osteosarcoma with the liposome with amphipathic polyethylene glycol(PEG) encapsulating anticancer agent or caffeine","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2827"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-06-14"},"publish_date":"2018-06-14","publish_status":"0","recid":"44716","relation_version_is_last":true,"title":["抗癌剤およびカフェイン封入リポゾームを用いた骨肉腫の治療とテーラーメイド化学療法"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:34:12.757364+00:00"}