{"created":"2023-07-27T06:51:21.812697+00:00","id":44755,"links":{},"metadata":{"_buckets":{"deposit":"8669e05a-dbff-4833-93cb-7c7ac0df0e2e"},"_deposit":{"created_by":18,"id":"44755","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"44755"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00044755","sets":["2812:2813:2826"]},"author_link":["77187","294"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-04","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"18p.","bibliographicVolumeNumber":"2006-2007","bibliographic_titles":[{"bibliographic_title":"平成19(2007)年度 科学研究費補助金 基盤研究(B) 研究成果報告書"},{"bibliographic_title":"2007 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ジアルジアの種内多型形成メカニズムとして地理的隔離と宿主特異性によるライフサイクルの隔離を想定し、多地域由来のジアルジアサンプルと同一地域の異なる宿主由来のジアルジアサンプルをインドネシアにおいて採取し、遺伝子型解析を実施した。具体的には2年間に4回のインドネシアの孤島地域でのフォールドワークを実施し、ヒト及び動物由来の計100サンプルを越えるジアルジアについて遺伝子型解析を実施した。その結果;1)ヒトのサンプルからは、いずれの地域においても高いレベルで遺伝子配列の保存されているassemblage A特にA2と、極めて多型に富みほとんどの検出配列がリファレンスとの完全一致をみないassemblage Bの亜型が同定された。2)従来イヌに特異的と考えられてきたassemblage CやDが、一部のヒトの糞便から検出された。3)少なくとも現在のジアルジアの遺伝子型の地理的分布には地域性は存在せず、地理的隔離の影響は求められない。4)Assemblage Bでは同一地域のヒトとヒト以外のほ乳類の両方から検出されたが、各宿主に分布するジアルジアの遺伝子多型にはそれぞれに優位な単系統のクラスターが認められたことから、何らかのライフサイクルの隔離機構が存在し各亜型に独自の変異蓄積が起こっている可能性が示唆された。つまりジアルジアの著しい種内多型の存在は、異なる宿主における部分的な隔離の継続といった同一地域での棲み分けをベースに成立・維持されていると考えられことから、世界中に分布する本原虫に対する新たな戦略構築には人獣共通感染症とこの微少隔離の制御の視点が重要である。","subitem_description_type":"Abstract"},{"subitem_description":"We aimed to determine the status of intra-species genetic diversity of Giardia intestinalis and the factors that contribute to the maintenance of population diversity in this species. Giardia samples collected from various mammals (dog, pig, mouse and human) i n different geographical sites(Indonesia, Nepal and Japan) were analyzed using multilocus markers (18S small subunit ribosomal RNA, glutamate dehydrogenase and triosephosphate isomerase genes). In the human samples, various sub-genotypes within assemblage A and/or B were clearly classified ; however, no site-specific distribution of genotypes was observed. We also confirmed that assemblages C and D, which have been recognized as dog-specific genotypes, were present in a few human samples in a rural area of Indonesia. A comparison of the determined sequences of human and animal isolates collected at the same site specifically confirmed the presence of each sub-assemblage cluster within assemblage B.\nTaken together with previous indications of assemblage-level host specificities in G. intestinalis, these results demonstrate that host specificities could be partly responsible for the assemblage levels of genetic polymorphism in this pathogen, however the diversity within each assemblage especially assemblage B seemed not in the case. Although the present geographical distributions and isolations of the pathogen were not thought to be a driving force of its genetic diversity since the distribution did not indicate any locality, we showed here the possibility that niche separations in a site might be related to the diversity formation. Niche separation may enable Giardia to accumulate the unique mutations in the genome and maintain novel diversity within the clonal population.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:18406007, 研究期間(年度):2006-2007","subitem_description_type":"Other"},{"subitem_description":"出典：「ジアルジアの種内変異に関する研究: インドネシアの孤立地域での遺伝子型分布」研究成果報告書　課題番号18406007\n  (KAKEN：科学研究費助成事業データベース（国立情報学研究所）)\n　　　本文データは著者版報告書より作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00051096","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学先進予防医学研究センター"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=30338024"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=30338024","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18406007/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18406007/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18406007/184060072007kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18406007/184060072007kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-06-18"}],"displaytype":"detail","filename":"ME-PR-TOKORO-S-kaken 2008-18p.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-TOKORO-S-kaken 2008-18p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/44755/files/ME-PR-TOKORO-S-kaken 2008-18p.pdf"},"version_id":"07c94e73-481b-4d7f-a157-c2743cb7ff85"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ジアルジアの種内変異に関する研究: インドネシアの孤立地域での遺伝子型分布","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ジアルジアの種内変異に関する研究: インドネシアの孤立地域での遺伝子型分布"},{"subitem_title":"Study of intra-species diversity in Giardia intestinalig Assessment of a genotype distribution in isolated islands of Indonesia","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2826"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-06-18"},"publish_date":"2018-06-18","publish_status":"0","recid":"44755","relation_version_is_last":true,"title":["ジアルジアの種内変異に関する研究: インドネシアの孤立地域での遺伝子型分布"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:24:10.664409+00:00"}