{"created":"2023-07-27T06:51:22.168507+00:00","id":44764,"links":{},"metadata":{"_buckets":{"deposit":"c7349cc7-4d6b-4a6e-8e3b-d99ba3d81463"},"_deposit":{"created_by":18,"id":"44764","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"44764"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00044764","sets":["2812:2813:2821"]},"author_link":["2653","77197"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-05-20","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2011-2012","bibliographic_titles":[{"bibliographic_title":"平成24(2012)年度 科学研究費補助金 挑戦的萌芽研究 研究成果報告書"},{"bibliographic_title":"2012 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"再生不良性貧血(AA)症例におけるHLA-Aアレル欠失血球陽性例の頻度を明らかにするため、抗HLA-Aアレル特異的抗体による検出感度を改良し、診断後間もない例を検索したところ、21症例中6例(28.6%)が陽性であり、欠失血球の全顆粒球中の割合は3.9% - 61.1%(中央値8.4%)と、既治療よりも低比率であった。一方、HLA-Aアレル欠失血球陽性AA患者の末梢血CD8陽性細胞を、HLA-B*40:02遺伝子導入K562 陽性細胞で刺激することにより、HLA-B*40:02導入K562のみを特異的に傷害する細胞傷害性T細胞(CTL)クローン(A6)を樹立した。","subitem_description_type":"Abstract"},{"subitem_description":"To determine the exact prevalence of HLA-A allele-lacking leukocytes (HLA-LLs) in patients with acquired aplastic anemia (AA), we improved the sensitivity of flow cytometry and examined peripheral blood leukocytes of patients with newly-diagnosed AA patients. HLA-LLs were detectable in 6 (28.6%) of 21 patients who were heterozygous with the HLA-A allele. The percentage of HLA-A allele-lacking granulocytes in the total granulocytes ranged from 3.9% to 61.1% (median 8.4%), which was lower than that in patients in remission after immunosuppressive therapy. Cytotoxic T cell (CTL) clones (A6) were successfully established from one of the patients possessing HLA-LLs by stimulating patient’s CD8+T cells with K562 cells transfected with HLA-B*40:02 gene. A6 CTL killed K562 in B*40:02 restricted manner but did not kill other cells including EB virus-transformed lymphoblastoid cell line and Jurkat T cell line transduced with B*40:02.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:23659486, 研究期間(年度):2011-2012","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「造血幹細胞におけるHLAアレル欠失現象を利用した再生不良性貧血自己抗原の同定」課題番号23659486\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23659486/23659486seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00051105","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学医薬保健研究域医学系"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=70217660"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=70217660","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659486/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659486/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23659486/23659486seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23659486/23659486seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-06-18"}],"displaytype":"detail","filename":"ME-PR-NAKAO-S-kaken 2013-4p.pdf","filesize":[{"value":"500.9 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-NAKAO-S-kaken 2013-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/44764/files/ME-PR-NAKAO-S-kaken 2013-4p.pdf"},"version_id":"0360552f-adfd-4ae3-b668-5003e250fc2c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"造血幹細胞におけるHLAアレル欠失現象を利用した再生不良性貧血自己抗原の同定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"造血幹細胞におけるHLAアレル欠失現象を利用した再生不良性貧血自己抗原の同定"},{"subitem_title":"Identification of auto-antigens in acquired aplastic anemia associated with clonal hematopoiesis by hematopoietic stem cells with uniparental disomy of chromosome 6p.","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2821"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-06-18"},"publish_date":"2018-06-18","publish_status":"0","recid":"44764","relation_version_is_last":true,"title":["造血幹細胞におけるHLAアレル欠失現象を利用した再生不良性貧血自己抗原の同定"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:37:28.530528+00:00"}