{"created":"2023-07-27T06:51:25.024303+00:00","id":44834,"links":{},"metadata":{"_buckets":{"deposit":"1edc36a4-4841-4ed0-858c-0a72291e64c3"},"_deposit":{"created_by":18,"id":"44834","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"44834"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00044834","sets":["2812:2813:2822"]},"author_link":["2881","20436"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5p.","bibliographicVolumeNumber":"2009-2011","bibliographic_titles":[{"bibliographic_title":"平成23(2011)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2011 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"EBV-HLHは著しい高サイトカイン血症と血球減少を特徴とし、急激な全身状態の悪化によりしばしば致死的経過をたどる。数時間の治療の遅れが不可逆的な臓器傷害を惹起することから、超早期の診断と治療的介入が必須の、救急疾患である。その本態が主としてCD8^+T細胞への選択的EBV感染と、感染クローンの強い活性化、選択的増殖であることは我々がすでに明らかにしている。したがってその診断にはTCR構造解析により特定のCD8^+T細胞クローンへのEBV感染を証明することが重要となる。しかし、このような高度に特殊な解析が可能な医療機関は多くなく、代替となるより簡便な検査法の開発が必要とされる。\n本研究ではEBV-HLH症例においてEBVが感染するCD8^+T細胞クローンの特徴について、特にその表面抗原発現プロフィールと、サイトカイン産生プロフィールを中心に解析した。さらにその結果をもとにして、簡便で迅速な診断プロトコールの提示を試みた。EBV-HLHではほぼ例外なく、特定のTCR Vβを発現するCD8^+T細胞にEBVが感染、CDR3サイズ分布解析によりそれらの細胞がクローン性増殖を示すことが明らかにされた。さらに、異常クローンはHLA-DR強陽性、CD5陰性の特徴を示した。その後の検討では、FHL2を背景としたEBV-HLHの場合はB細胞にEBVが感染、多クローン性にCD5陰性CD8^+T細胞が増殖することも明らかにされ、診断の際に留意すべきであると考えられた。","subitem_description_type":"Abstract"},{"subitem_description":"EBV-HLH is characterized by extreme cytokinemia and cytopenia, frequently leading to aggressive deterioration and fatal outcome. It is of paramount importance to make correct diagnosis and start therapeutic intervention as soon as possible, as the delay in the treatment may result in irreversible damage to vital organs within several hours. We now understand that the essential pathogenesis of EBV-HLH is selective activation and expansion of an ectopically EBV-infected CD8^+T cell clone. Thus the definitive diagnosis of EBV-HLH can be made by identifying the target of EBV infection and by proving the clonality of the EBV-infected cells.\nIn this study, we analyzed the profiles of surface antigen expression of the infected CD8^+T cells and serum cytokines. With these results, we tried to establish a simplified method to establish parameters for the early diagnosis of EBV-HLH. As was expected, EBV infection in most cases of EBV-HLH was confined to a single clone of CD8+T cells, expressing a particular TCR Vβ. CDR3 size distribution analysis indicates that these cells are of a single clone. The abnormal clones exhibited high levels of HLA-DR and decreased levels of CD5. Therefore, EBV-infected cells were identifiable as HLA-DR^++CD5^-cell population within CD8^+T cells. Further analysis revealed that similar cell population was observed in patients with FHL2 during acute viral infection, including EBV.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:21591353, 研究期間(年度):2009-2011","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「早期治療介入のための新しいEBM関連リンパ増殖性疾患診断指標の確立」課題番号21591353\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-21591353/21591353seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00051173","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学医薬保健研究域医学系"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=40210281"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40210281","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591353/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591353/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-21591353/21591353seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-21591353/21591353seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-06-22"}],"displaytype":"detail","filename":"HO-PR-YACHIE-A-kaken 2012-5p.pdf","filesize":[{"value":"240.6 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"HO-PR-YACHIE-A-kaken 2012-5p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/44834/files/HO-PR-YACHIE-A-kaken 2012-5p.pdf"},"version_id":"79341909-67d2-4f74-9763-d190d8736d33"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"早期治療介入のための新しいEBM関連リンパ増殖性疾患診断指標の確立","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"早期治療介入のための新しいEBM関連リンパ増殖性疾患診断指標の確立"},{"subitem_title":"Establishment of novel diagnostic parameters for the early therapeutic intervention of EBV-associated lymphoproliferative diseases","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2822"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-06-22"},"publish_date":"2018-06-22","publish_status":"0","recid":"44834","relation_version_is_last":true,"title":["早期治療介入のための新しいEBM関連リンパ増殖性疾患診断指標の確立"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T13:27:58.308820+00:00"}