{"created":"2023-07-27T06:51:25.287500+00:00","id":44840,"links":{},"metadata":{"_buckets":{"deposit":"168a2b3b-4a58-41a8-a6e0-d3980f35db54"},"_deposit":{"created_by":18,"id":"44840","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"44840"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00044840","sets":["2812:2813:2840"]},"author_link":["2881","20436"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"9p.","bibliographicVolumeNumber":"1992-1993","bibliographic_titles":[{"bibliographic_title":"平成5(1993)年度 科学研究費補助金 一般研究(C) 研究成果報告書"},{"bibliographic_title":"1993 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"末梢血好酸球にはCD11b抗原が構成的に発現、好酸球数の多少に関係なく種々のアレルギー疾患において、正常対照よりもその程度は強かった。CD69は末梢血好酸球には殆んど発現せず、炎症局所に浸潤する好酸球に強く発現していた。ただし、好酸球数の著明に増多するHES例では、末梢血好酸球の一部が強くCD69を発現した。CD69陽性好酸球は、陰性好酸球に比し、脱顆粒傾向が強い場合や、過分葉傾向が強い場合が認められた。in vitroでの検討では、好酸球の分化・増殖に関与するとされる、インターロイキン3,インターロイキン5さらにGM-CSFの添加、培養でCD69発現が速やかに誘導され、生体内における好酸球表面抗原の発現はこれらサイトカイン産生を反映していると考えられた。以上の事実より、好酸球増多性疾患においては、光顕的・電顕的好酸球形態の観察のみならず、CD11b,CD69等の好酸球表面抗原発現を検索することが極めて重要であると考えられた。このような活性化抗原発現が、炎症細胞としての好酸球機能発現にどのように関与しているかは不明な点が多く、今後の顆題として残される。\n一方、新生児ならびに成人CD4^+T細胞のスーパー抗原応答性を比較検討した。成人CD4^+T細胞に比し、新生児CD4^+T細胞のスーパー抗原応答性は極めて良好であり、用いたスーパー抗原全てに対して新生児CD4^+T細胞の方が極めて良好な^3H-TdR取り込みを示した。このようなT細胞の高応答性は、持続的かつ著明なLL-2産生と、スーパー抗原特異的なT細胞V_βレパートリーの選択的増殖によると考えられた。この時期のアレルギー性疾患の病態像の特徴とスーパー抗原高反応性は、極めて興味深く、今後の検討が重要である。","subitem_description_type":"Abstract"},{"subitem_description":"Rapid induction of CD69 and upregulation of CD11b on eosinophil surface was observed after incubation of heparinized whole blood cells with IL-3, IL-5 or GM-CSF.Peak expression of CD69 was reached assoon as 2 hours after simulation, whereas CD11b expression reached the peak several hours after simulation. Surface expression of eosinophil membrane antigen CD11b and CD69 was also examined in vivo. CD11b is expressed constitutively on normal eosinophils in the peripheral blood. Little, if any CD69 expression was detectable on circulating eosinophils from normal or allergic patients, including bronchial asthma and atopic dermatitis. CD69 expression was upregulated on eosinophils from local inflammatory sites, such as pericardial efusion of eosinophilic carditis, or nasal discharge from allergic rhinitis.\nA significant proportion of circulating eosinophils from hypereosinophilic syndrome expressed CD69 on the surface, indicating that these eosinophils are recently activated by exposure to co ncentrated eosinophilopoietic cytokine, such as IL-3, IL-5 and GM-CSF.Tese rapid induction of eosinophil surface antigens may be functionally significant during the induction of eosinophilic inflammatory lesions as represented by bronchial asthma and atopic dermatitis.\nCharacteristics of neonatal T lymphocyte activation and cytokine production was analyzed by superantigeninduced T cell activation.Antigen-responsiveness and cytokine profiles of neonatal and infantile T lymphocytes may have important relatiionship with the characteristic pathology of allergic disorders during this period of life. Neonatal CD4^+ T lymphocyte proliferated vigorously to superatigen stimulation. The much higher proliferative resopnses of neonatal T cells than adult memory T cells was shown to be based on the greater and prolonged production of IL-2, together with the selective proliferation of Vbeta specific T cell proliferation of neonatal T cells. The enhance antigen responsiveness of neonatal or naive T cells to superantigen may be closely related to the occurrence of febrile inflammatory illness associated with desquamative skin lesions, characteristic during early childhood. It is not known if these characteristics of neonatal T cell responsiveness is related to the pathogenesis of allergic disorders during early childhood.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:04670583, 研究期間(年度):1992-1993","subitem_description_type":"Other"},{"subitem_description":"出典：「全血法を用いた好酸球表面抗原発現の検討」研究成果報告書　課題番号04670583\n(KAKEN：科学研究費助成事業データベース（国立情報学研究所）)\n　　　本文データは著者版報告書より作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00051179","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学医薬保健研究域医学系"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=40210281"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40210281","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04670583/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04670583/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-04670583/046705831993kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-04670583/046705831993kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-06-22"}],"displaytype":"detail","filename":"HO-PR-YACHIE-A-kaken 1994-9p.pdf","filesize":[{"value":"501.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"HO-PR-YACHIE-A-kaken 1994-9p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/44840/files/HO-PR-YACHIE-A-kaken 1994-9p.pdf"},"version_id":"bc76b494-12b9-47e5-8385-5e9972f57831"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"全血法を用いた好酸球表面抗原発現の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"全血法を用いた好酸球表面抗原発現の検討"},{"subitem_title":"Flowcytometric Analysis of Eosinophil Surface Antigens Utlizing","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2840"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-06-22"},"publish_date":"2018-06-22","publish_status":"0","recid":"44840","relation_version_is_last":true,"title":["全血法を用いた好酸球表面抗原発現の検討"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:46:47.842949+00:00"}