{"created":"2023-07-27T06:51:25.681760+00:00","id":44849,"links":{},"metadata":{"_buckets":{"deposit":"0a3bfbae-4af9-4ae6-9150-5a039e49ec85"},"_deposit":{"created_by":18,"id":"44849","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"44849"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00044849","sets":["2812:2813:2833"]},"author_link":["77348","210"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5p.","bibliographicVolumeNumber":"1998-2000","bibliographic_titles":[{"bibliographic_title":"平成12(2000)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2000 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"高齢者やアルツハイマー病患者の脳内にみられるアルツハイマー型病理変化の形成に関与する因子を解明する目的で以下の研究をした。\n(1)臨床的・神経病理学的検索:アルツハイマー病(AD)を含む60歳以上の200剖検例を対象とし、臨床データ、脳病変の検討(アミロイドβ蛋白やリン酸化タウに対する抗体、ミクログリアやアストロサイトなどのマーカーに対する免疫染色)を行った。AD型病理変化(老人斑、神経原線維変化、脳アミロイドアンギオパチーCAA)について定量的に評価した。\n(2)遺伝的危険因子の検索:患者血液あるいは凍結脳からDNAを抽出後、presenilin-1(PS1)遺伝子、α1-antichymotrypsin(ACT)遺伝子、butyrylcholinesterase(BChE)遺伝子、α2-マクログロブリン(A2M)遺伝子、paraoxonase(PON)遺伝子多型を検索した。\n(3)アルツハイマー型神経病理変化と遺伝子多型との関連を検討した。\n(4)アミロイド細線維形成実験:脳アミロイド細線維形成に影響を与える因子を検索するために、アミロイドβ蛋白のin vitroでの実験系について検討した。\nその結果、以下のような研究結果を得た。\n(1)PS-1遺伝子多型はCAAと関連したが、ADの発症、老人斑、神経原線維変化とは関連しなかった。\n(2)ACT遺伝子多型はADの発症や老人斑、神経原線維変化とは関連しなかったが、AD脳におけるCAAの程度と関連した。\n(3)BChE遺伝子多型(K variant)はADやCAAとは関連しなかったが、老人斑、神経原線維変化の程度と関連した。\n(4)A2MおよびPON遺伝子多型はAD、老人斑、神経原線維変化、CAAのいずれとも関連しなかった。\n(5)アミロイドβ蛋白の重合・線維形成ばかりでなく、分解・脱重合をモニターできるin vitroの系を開発した。\n以上の研究成果により、既に確立したADの危険因子であるアポリポタンパクE遺伝子型以外に、老人斑、神経原線維変化、CAAなどのAD型神経病理変化の新たな危険因子が明らかになった。","subitem_description_type":"Abstract"},{"subitem_description":"To investigate risk factors for formation of Alzheimer type neuropathological changes such as senile plaques (SP), neurofibrillary tangles (NFT), and cerebral amyloid angiopathy (CAA), we performed pathological, immunohistochemical, and molecular genetic studies in the elderly Japanese individuals including patients with Alzheimer disease (AD). The results were summarized as follows :\n1) A polymorphism in the intron 8 of presenilin 1 gene was associated with CAA, but not with AD, SP, or NFT.\n2) A polymorphism in the signal peptide of alpha 1-antichymotrypsin (ACT) was associated with CAA in AD brains, but not with AD, SP, or NFT.\n3) A polymorphism (K variant) in butyrylcholinesterase gene was associated with SP and NFT, but not with AD and CAA.\n4) Polymorphisms in alpha2-macroglobulin and paraoxonase genes were not associated with AD, SP, NFT, or CAA.\nIn addition, we developed an in vitro system in which dissolution of beta amyloid fibrils as well as fibril formation of beta peptide can be monitored. The system would be useful to identify factors which influence amyloid fibrillogenesis in the brain.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:10670579, 研究期間(年度):1998-2000","subitem_description_type":"Other"},{"subitem_description":"出典：「アルツハイマー型神経病理変化形成の危険因子に関する研究」研究成果報告書　課題番号10670579\n(KAKEN：科学研究費助成事業データベース（国立情報学研究所）)\n　　　本文データは著者版報告書より作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00051188","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学医薬保健研究域医学系"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=80191336"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=80191336","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670579/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670579/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-10670579/106705792000kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-10670579/106705792000kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-06-22"}],"displaytype":"detail","filename":"ME-PR-YAMADA-M-kaken 2001-5p.pdf","filesize":[{"value":"243.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-YAMADA-M-kaken 2001-5p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/44849/files/ME-PR-YAMADA-M-kaken 2001-5p.pdf"},"version_id":"a14647b5-45ae-4019-844a-2c26e4e5940d"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"アルツハイマー型神経病理変化形成の危険因子に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"アルツハイマー型神経病理変化形成の危険因子に関する研究"},{"subitem_title":"Study on risk factors for Alzheimer type neuropathological-changes","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2833"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-06-22"},"publish_date":"2018-06-22","publish_status":"0","recid":"44849","relation_version_is_last":true,"title":["アルツハイマー型神経病理変化形成の危険因子に関する研究"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:46:15.006509+00:00"}