{"created":"2023-07-27T06:51:35.169073+00:00","id":45065,"links":{},"metadata":{"_buckets":{"deposit":"29e66597-2fd7-4d56-b9e3-9fb8b02d9d86"},"_deposit":{"created_by":18,"id":"45065","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"45065"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00045065","sets":["2812:2813:2821"]},"author_link":["2407","20219"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-04-25","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2011-2012","bibliographic_titles":[{"bibliographic_title":"平成24(2012)年度 科学研究費補助金 若手研究(B) 研究成果報告書"},{"bibliographic_title":"2012 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"近年、免疫反応を抑制する作用を持つIL-10産生制御性B細胞が発見され、免疫疾患において非常に重要であることが明らかとなった。制御性B細胞の分化・増殖にはB細胞の表面マーカーであるCD19およびそのシグナル伝達(PI3Kシグナル)が重要であることが示唆されている。本研究では、PI3Kシグナルによる制御性B細胞の制御機構を解析した。本研究にてPI3Kの阻害剤が、用量依存性にIL-10産生を阻害しりことを明らかとした。さらにPI3K/AKTのinhibitorであるPTENのB細胞特異的欠損マウスを作成し、同マウスにてIL-10産生制御性B細胞が著増していることを確認した。B細胞特異的PTEN欠損マウスではContact hypersensitivity反応の著明な低下が認められた。以上より、IL-10産生制御性B細胞の制御機構において、PI3K/AKT経路が重要であることが明らかとなった。","subitem_description_type":"Abstract"},{"subitem_description":"IL-10 producing regulatory B (Breg) cells negatively regulates autoimmune disease and inflammation, such as contact hypersensitivity (CHS). CD19 expression is critical for Breg cells development, since CD19 loss results in decreased number of Breg cell and increased reactivity of CHS. However, molecular mechanism of Breg cell development remains poorly understood. CD19 downstream signal is depend on the activation of phosphoinositide 3-kinases (PI3K)-AKT pathways, while phosphatase and tensin homologue (PTEN) attenuates PI3K-AKT pathways. In this study, we investigated the role of PI3K-AKT pathway in the development of Breg cells. The effect of PI3K-AKT pathway inhibitors on Breg cell development was examined in vitro. B cell-specific PTEN deficient mice, which exhibit aberrant activation of PI3K-AKT pathway in B cell, were generated using Cre-loxp system. CHS severity in B cell-specific PTEN deficient mice was investigated. The current studies demonstrate that PI3K-AKT pathway inhibitors reduced Breg cells in vitro in dose-dependent manner. However, PTEN inhibitor had no effect on Breg cells. Breg cell number and frequency were significantly increased in various organs of B cell-specific PTEN deficient mice when compared with wild-type mice. Furthermore, CHS response was significantly diminished in B cell-specific PTEN deficient mice when compared with wild-type mice. Thus, PI3K-AKTpathway positively regulates Breg cell development, while PTEN negatively regulates it. PI3K-AKT pathway in B cell is critical for Breg cell development and could be a potent therapeutical target.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:23791260, 研究期間(年度):2011-2012","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「制御性B細胞におけるPI3Kシグナルによる制御機構の解析」課題番号23791260\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23791260/23791260seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学附属病院","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00051404","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学附属病院"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=60432126"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=60432126","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791260/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791260/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23791260/23791260seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23791260/23791260seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-07-05"}],"displaytype":"detail","filename":"HO-PR-MATSUSHITA-T-kaken 2013-4p.pdf","filesize":[{"value":"478.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"HO-PR-MATSUSHITA-T-kaken 2013-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/45065/files/HO-PR-MATSUSHITA-T-kaken 2013-4p.pdf"},"version_id":"0b6af798-a852-4781-9a01-c004167f128c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"制御性B細胞におけるPI3Kシグナルによる制御機構の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"制御性B細胞におけるPI3Kシグナルによる制御機構の解析"},{"subitem_title":"Analysis of PI3K signaling role in regulatory B cells","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2821"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-07-05"},"publish_date":"2018-07-05","publish_status":"0","recid":"45065","relation_version_is_last":true,"title":["制御性B細胞におけるPI3Kシグナルによる制御機構の解析"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:37:14.909273+00:00"}