{"created":"2023-07-27T06:51:40.389911+00:00","id":45185,"links":{},"metadata":{"_buckets":{"deposit":"7d7aa4c7-9e7a-4479-b33a-e68126b0f1da"},"_deposit":{"created_by":18,"id":"45185","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"45185"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00045185","sets":["2812:2813:2817"]},"author_link":["22250","78637"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-05-22","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5p.","bibliographicVolumeNumber":"2014-04-01 - 2017-03-31","bibliographic_titles":[{"bibliographic_title":"平成28(2016)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2016 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"間葉系幹細胞（MSC）においてp53蛋白は骨芽細胞分化や脂肪細胞分化を抑制する。同一個体からALT/WDL組織と正常脂肪組織を採取し、MSCを分離培養し、骨芽細胞分化能と脂肪細胞分化能を比較した。脂肪分化能においては、我々の予想に反して、正常脂肪由来MSCにおいてより多くの脂肪滴が認められた。対照的に、骨芽細胞分化能においては、ALT/WDL由来MSCでより高い分化能を認めた。ALP染色に基づく骨芽細胞分化能の差異はMDM2特異的阻害薬Nutlin-3濃度が上昇するにつれて減少した。ALT/WDL由来MSCにおけるMDM2遺伝子の増幅がより高い骨芽細胞分化能をもたらしている可能性が示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"We compared osteogenic and adipogenic differentiation potency between MSCs isolated and cultured from normal adipose tissues and atypical lipomatous tumor/well-differentiated liposarcoma (ALT/WDL) from the same patients. We found greater osteogenic differentiation potency in ALT/WDL-derived MSCs than in normal fat-derived MSCs. In adipogenic differentiation, contrary to our expectations, more fatty acid droplets were observed in normal fatderived MSCs than in ALT/WDL-derived MSCs. In osteogenic differentiation, as expected, MSCs derived from ALT/WDL had higher differentiation potency than normal fat-derived MSCs. The gap of osteogenic differentiation potency based on ALP staining between ALT/WDL-derived MSCs and normal fat-derived MSCs disappeared as Nutlin-3 concentration increased, suggesting that greater osteogenic differentiation could be caused by amplied MDM2 in ALT/WDL.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:26462271, 研究期間(年度):2014-04-01 - 2017-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「脂肪由来幹細胞を応用した骨軟部腫瘍切除後組織欠損の再生医療と蛍光イメージング評価」研究成果報告書　課題番号26462271\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26462271/26462271seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学附属病院整形外科","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00051524","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=80507054"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=80507054","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26462271/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26462271/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26462271/26462271seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26462271/26462271seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-07-05"}],"displaytype":"detail","filename":"HO-PR-HAYASHI-K-kaken 2017-5p.pdf","filesize":[{"value":"301.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"HO-PR-HAYASHI-K-kaken 2017-5p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/45185/files/HO-PR-HAYASHI-K-kaken 2017-5p.pdf"},"version_id":"c06fb2ae-7099-4b9f-b306-541d552f0149"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"脂肪由来幹細胞を応用した骨軟部腫瘍切除後組織欠損の再生医療と蛍光イメージング評価","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"脂肪由来幹細胞を応用した骨軟部腫瘍切除後組織欠損の再生医療と蛍光イメージング評価"},{"subitem_title":"Regeneration using adipose-derived stem cells and fluorescent imaging evaluation for a defect after bone and soft tissue tumor surgery","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2817"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-07-05"},"publish_date":"2018-07-05","publish_status":"0","recid":"45185","relation_version_is_last":true,"title":["脂肪由来幹細胞を応用した骨軟部腫瘍切除後組織欠損の再生医療と蛍光イメージング評価"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T11:31:27.818540+00:00"}