{"created":"2023-07-27T06:51:42.123648+00:00","id":45224,"links":{},"metadata":{"_buckets":{"deposit":"8a5123b2-01c3-480e-a0fb-a6e827d1a0d1"},"_deposit":{"created_by":18,"id":"45224","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"45224"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00045224","sets":["2812:2813:2820"]},"author_link":["70371","337"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-05-29","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5p.","bibliographicVolumeNumber":"2011-2013","bibliographic_titles":[{"bibliographic_title":"平成25(2013)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2013 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"脂肪組織由来間質細胞への遺伝子導入による肝細胞への分化誘導を試みる研究を行った。GFPタグ付HNF4a、HNF1b発現プラスミド(pCMV6-HNF4a-GFP, pCMV-6HNF1b-GFP)をエレクトロポレーション法にて導入した。導入後15日目の細胞を回収し、リアルタイムPCRにて遺伝子発現解析を行った。pCMV6-HNF4a-GFP導入細胞ではHNF4aの発現を認めたが、Albumin発現は見られなかった。pCMV6-HNF1b-GFP導入細胞は、HNF1bの発現、Albuminの発現双方認められなかった。脂肪組織由来間質細胞への遺伝子導入による肝細胞分化には、改良が必要であった。","subitem_description_type":"Abstract"},{"subitem_description":"The experimental trial of differentiation of adipose-tissue derived stromal cells to hepatocyte by means of gene transduction was conducted. The pCMV6-HNF4a-GFP expressing GFP-tagged HNF4a and the pCMV6-HNF1b-GFP expressing GFP-tagged HNF1b was transduced to adipose tissue-derived stromal cells by electroporation method. Cells at day 15 after transduction was assessed for gene expression by real-time detective PCR. Cells transduced with pCMV6-HNF4a-GFP expressed HNF4a, however, did not express albumin. Cells transduced with pCMV6-HNF1b-GFP neither expressed HNF1b nor albumin. Improvement is required for differentiation of adipose tissue derived stromal cells by electroporation method.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:23590968, 研究期間(年度):2011-2013","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「遺伝子導入による間葉系幹細胞の肝修復再生能プログラミングと肝再生療法への応用」課題番号23590968\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23590968/23590968seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00051563","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学医薬保健研究域医学系"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=80401925"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=80401925","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590968/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590968/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23590968/23590968seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23590968/23590968seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-07-09"}],"displaytype":"detail","filename":"ME-PR-SAKAI-Y-kaken 2014-5p.pdf","filesize":[{"value":"102.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-SAKAI-Y-kaken 2014-5p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/45224/files/ME-PR-SAKAI-Y-kaken 2014-5p.pdf"},"version_id":"9eaaba12-e6fc-4224-8f12-3e1eb1b885f3"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"遺伝子導入による間葉系幹細胞の肝修復再生能プログラミングと肝再生療法への応用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"遺伝子導入による間葉系幹細胞の肝修復再生能プログラミングと肝再生療法への応用"},{"subitem_title":"Gene transfer programming of mesenchymal stem cells for repairing and regeneration of the liver, and its applocation for liver regeneration therapy","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2820"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-07-09"},"publish_date":"2018-07-09","publish_status":"0","recid":"45224","relation_version_is_last":true,"title":["遺伝子導入による間葉系幹細胞の肝修復再生能プログラミングと肝再生療法への応用"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T09:18:43.808550+00:00"}