{"created":"2023-07-27T06:51:44.313887+00:00","id":45276,"links":{},"metadata":{"_buckets":{"deposit":"be2f661a-b222-4507-acd1-8dec6cb284ce"},"_deposit":{"created_by":18,"id":"45276","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"45276"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00045276","sets":["2812:2813:2816"]},"author_link":["2034","78758"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-05-28","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2015-04-01 - 2018-03-31","bibliographic_titles":[{"bibliographic_title":"平成29(2017)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2017 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamazaki, Hiroto"}],"nameIdentifiers":[{"nameIdentifier":"78758","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50361994","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50361994"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"TGF-β副受容体であるGPIアンカー膜蛋白CD109が、PIGA遺伝子変異造血幹前駆細胞（HSPC）の優先的活性化に関与しているか否かを明らかにするため、白血病細胞株TF-1のCD109をノックアウト（KO）し、野生型との間で、TGF-βに対する感受性を比較した。その結果、CD109KO TF-1細胞では野生型TF-1細胞に比べてTGF-βによるリン酸化SMAD2の誘導が低下し、TGF-βによる細胞増殖の抑制が起こりにくいことが明らかになった。PIGA遺伝子変異HSPCは、TGF-βの作用増強を担うCD109の欠失によりTGF-βによる抑制を免れて造血に寄与しやすくなることが示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"To determine whether a TGF-beta (b) co-receptor CD109 on hematopoietic stem progenitor cells (HSPCs) play a role in the preferential commitment of PIGA mutant HSPCs in patients with immune-mediated bone marrow failure (BMF), we established CD109-knock out (KO) TF-1, a GM-CSF-dependent myeloid leukemia cell line, using the CRIPR/Cas9 system, and compared the sensitivity of CD109KO TF-1 cells to TGF-b to that of wild-type (WT) TF-1 cells. The TGF-b treatment induced pSMAD2 in CD109KO TF-1 cells to a significantly lesser degree than in WT TF-1 cells. The proliferation of CD109KO TF-1 was inhibited by TGF-b also to a lesser degree than WT TF-1 cells. The results suggest that CD109, a glycosylphosphatidylinositol anchor protein, plays an enhancing role in the TGF-b signaling in HSPCs, and the lack of CD109 may make the HSPCs less sensitive to TGF-b, leading to the preferential commitment of the PIGA mutant HSPCs in immune-mediated BMF, in which TGF-b suppresses activation of WT HSPCs.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:15K09496, 研究期間(年度):2015-04-01 - 2018-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「PIGA遺伝子変異造血幹細胞の選択的増殖におけるCD109分子とTGF-βの役割」課題番号15K09496\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K09496/15K09496seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学附属病院輸血部","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00051613","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=50361994"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=50361994","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K09496/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K09496/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K09496/15K09496seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K09496/15K09496seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山崎, 宏人"}],"nameIdentifiers":[{"nameIdentifier":"2034","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50361994","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50361994"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-04-19"}],"displaytype":"detail","filename":"HO-PR-YAMAZAKI-H-kaken 2018-4p.pdf","filesize":[{"value":"169.1 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"HO-PR-YAMAZAKI-H-kaken 2018-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/45276/files/HO-PR-YAMAZAKI-H-kaken 2018-4p.pdf"},"version_id":"a58bf2c4-48c9-4f87-839d-ba269ed76d8b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"PIGA遺伝子変異造血幹細胞の選択的増殖におけるCD109分子とTGF-βの役割","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"PIGA遺伝子変異造血幹細胞の選択的増殖におけるCD109分子とTGF-βの役割"},{"subitem_title":"The role of CD109 and TGF-beta for the preferential commitment of PIGA mutant HSPCs in immune-mediated bone marrow failure","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2816"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-04-19"},"publish_date":"2019-04-19","publish_status":"0","recid":"45276","relation_version_is_last":true,"title":["PIGA遺伝子変異造血幹細胞の選択的増殖におけるCD109分子とTGF-βの役割"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T05:32:27.716319+00:00"}