{"created":"2023-07-27T06:51:44.358538+00:00","id":45277,"links":{},"metadata":{"_buckets":{"deposit":"d8ecfccf-b35a-4c5b-8622-14419da0c8da"},"_deposit":{"created_by":18,"id":"45277","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"45277"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00045277","sets":["2812:2813:2821"]},"author_link":["2034","78758"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-05-20","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5p.","bibliographicVolumeNumber":"2010-2012","bibliographic_titles":[{"bibliographic_title":"平成24(2012)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2012 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"PNH型血球は、骨髄不全における免疫病態のマーカーとして有用であるものの、陽性細胞が少数の場合には判定に迷うことが多い。このため研究開始時には、各ドットにおける蛍光強度の数学的定量化を試みたが、日常臨床への適応は困難であった。抗GPIアンカー膜蛋白抗体の代わりにFLAREを用いることにより、PNH型血球の正確な定量化が達成された。PNH型血球が陰性でも免疫抑制療法が奏効する例があることから、さらに良いマーカーを探索したところ、血漿トロンボポエチン(TPO)値の高値が免疫病態と相関しており、PNH型血球陽性例を包含していることが明らかになった。したがって血漿TPOの測定は骨髄不全の病態診断に有用であることが示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"Although detection of PNH-type cells is useful as a marker for immune pathophysiology of bone marrow failure, it is often difficult when the percentage of PNH-type cells is less than 0.1%. We established an objective method of quantifying PNH-type with mathematical analysis of the fluorescent intensity of GPI-anchored proteindots on the flow cytometry dotgram, but the method was difficult to generalize. The use of fluorescent labeled aerolysin (FLARE) instead of monoclonal antibodies greatly improved the accuracy of detecting PNH-type cells. However, some patients without increased PNH-type cells respond to immunosuppressive therapy. To identify a better marker for immune pathophysiology of bone marrow failure, we examined a correlation of plasma thrombopoietin (TPO) levels with pathophysiology of bone marrow failure. Bone marrow failure patients showing TPO levels>320 pg/ml proved to have benign diseases with immune pathophysiology and virtually included all patients with increased PNH-type cells. The measurement of plasma TPO levels is therefore useful for diagnosing pathophysiology of bone marrow failure.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:22591032, 研究期間(年度):2010-2012","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「微少PNH型血球陽性骨髄不全診断システムの開発」課題番号22591032\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-22591032/22591032seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00051614","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学附属病院輸血部"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=50361994"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=50361994","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591032/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591032/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-22591032/22591032seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-22591032/22591032seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-07-09"}],"displaytype":"detail","filename":"HO-PR-YAMAZAKI-H-kaken 2013-5p.pdf","filesize":[{"value":"349.8 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"HO-PR-YAMAZAKI-H-kaken 2013-5p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/45277/files/HO-PR-YAMAZAKI-H-kaken 2013-5p.pdf"},"version_id":"352f0297-da45-4aed-b379-0ef9b878ba16"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"微少PNH型血球陽性骨髄不全診断システムの開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"微少PNH型血球陽性骨髄不全診断システムの開発"},{"subitem_title":"Development of the diagnostic system of bone marrow failure with a minor population of PNH-phenotype cells","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2821"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-07-09"},"publish_date":"2018-07-09","publish_status":"0","recid":"45277","relation_version_is_last":true,"title":["微少PNH型血球陽性骨髄不全診断システムの開発"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:37:05.990485+00:00"}