{"created":"2023-07-27T06:51:46.663951+00:00","id":45333,"links":{},"metadata":{"_buckets":{"deposit":"247313f4-0829-4204-9c6b-02a9d9f3480f"},"_deposit":{"created_by":18,"id":"45333","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"45333"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00045333","sets":["2812:2813:2816"]},"author_link":["78829","195"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-06-01","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5p.","bibliographicVolumeNumber":"2015-04-01 - 2018-03-31","bibliographic_titles":[{"bibliographic_title":"平成29(2017)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2017 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"紫外線による塩基損傷がヌクレオチド除去修復（NER）により二次的なDNA損傷に変換されるメカニズムの解明を目指すとともに、生体内での二次的DNA損傷の生成について検討した。その結果、Exo1がNER反応のssDNAギャップ中間体を拡大していることが明らかとなり、ATR経路を活性化することがわかった。また、Exo1がDSBに対するシグナリング経路にも影響を与えることや、細胞死の防御に寄与することを見出した。そしてマウス皮膚組織における諸反応について解析を行い、表皮の基底細胞層においてNER依存的なDNA損傷応答反応が起きることを示し、生体内でも二次的DNA損傷が生成することを示唆した。","subitem_description_type":"Abstract"},{"subitem_description":"In quiescent cells, nucleotide excision repair (NER) process generates multiple types of secondary DNA damage. However, the mechanism of secondary DNA damage formation and their biological meanings are unclear. In this study, we have examined the possible involvement of exonuclease 1 (Exo1) in the processing of ssDNA gap intermediate during NER. We found that Exo1 plays an important role in the processing of NER-induced ssDNA gap intermediates and protects UV-induced cell death in quiescent cells. In addition, we could detect several NER-dependent DNA damage responses in mouse skin, such as H2AX phosphorylation and ATM activation, indicating that NER-dependent formation of secondary DNA damage indeed occurs in vivo as well.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:15K00534, 研究期間(年度):2015-04-01 - 2018-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「休止期のヌクレオチド除去修復により生じる二次的DNA損傷とゲノム恒常性維持機構」課題番号15K00534\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K00534/15K00534seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域薬学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00051670","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=80345595"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=80345595","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K00534/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K00534/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K00534/15K00534seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K00534/15K00534seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-04-19"}],"displaytype":"detail","filename":"PH-PR-WAKASUGI-M-kaken 2018-5p.pdf","filesize":[{"value":"780.1 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"PH-PR-WAKASUGI-M-kaken 2018-5p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/45333/files/PH-PR-WAKASUGI-M-kaken 2018-5p.pdf"},"version_id":"039f8064-c426-47a2-97a8-4bc568e4b8b4"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"休止期のヌクレオチド除去修復により生じる二次的DNA損傷とゲノム恒常性維持機構","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"休止期のヌクレオチド除去修復により生じる二次的DNA損傷とゲノム恒常性維持機構"},{"subitem_title":"Analysis of secondary DNA damage elicited by nucleotide excision repair and the genome maintenance mechanism in mammalian quiescent cells","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2816"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-04-19"},"publish_date":"2019-04-19","publish_status":"0","recid":"45333","relation_version_is_last":true,"title":["休止期のヌクレオチド除去修復により生じる二次的DNA損傷とゲノム恒常性維持機構"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:59:00.235132+00:00"}