{"created":"2023-07-27T06:51:46.707743+00:00","id":45334,"links":{},"metadata":{"_buckets":{"deposit":"a5c7aafd-b499-46b8-977a-93782a5246a0"},"_deposit":{"created_by":18,"id":"45334","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"45334"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00045334","sets":["2812:2813:2819"]},"author_link":["78829","195"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-06-01","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5p.","bibliographicVolumeNumber":"2012-04-01 - 2015-03-31","bibliographic_titles":[{"bibliographic_title":"平成26(2014)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2014 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"休止期のヌクレオチド除去修復に依存して活性化するシグナル伝達経路に着目し、その初期シグナルと生物学的意義の解明を目的として実験を行なった。その結果、その活性化はヌクレオチド除去修復の反応中間体を介して生じる２種類の二次的なDNA損傷に起因しており、致死的な作用が強い二本鎖切断が生じることを明らかにした。また、電離放射線感受性として知られる毛細血管拡張性小脳失調症患者由来の細胞が、休止期においては紫外線にも高感受性であることを示した。さらに、生体から単離した休止期もしくは休止期様の細胞について検討し、同調した培養細胞で観察されるヌクレオチド除去修復依存的な反応が生じることを明らかにした。","subitem_description_type":"Abstract"},{"subitem_description":"In this study, we tried to clarify the nucleotide excision repair (NER)-related DNA structure(s) activating the signal transduction pathways and their biological impacts. We found that, under the quiescent condition, DSB (DNA double-strand break) is generated in an NER-dependent manner, in addition to the predicted single-stranded regions. In NER-proficient cells arrested in G0 phase, UV exposure activates ATM signaling pathway, which leads to the accumulation of DSV-related factors. Importantly, A-T cells are more sensitive to UV compared with normal cells when exposed under quiescent but not exponentially growing condition. Finally, we show that the NER-dependent H2AX phosphorylation is also observed in peripheral T lymphocytes and hematopoietic stem cells from mice. These all results suggest that in vivo quiescent cells may suffer from the mixed types of DNA lesions such as ssDNA gaps and DSB after UV or chemical exposure generating NER substrates.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号: 24510068, 研究期間 (年度): 2012-04-01 – 2015-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「休止期においてNER依存的に活性化するDNA損傷応答機構の解析」課題番号24510068\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24510068/24510068seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域薬学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00051671","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=80345595"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=80345595","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24510068/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24510068/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24510068/24510068seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24510068/24510068seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-07-13"}],"displaytype":"detail","filename":"PH-PR-WAKASUGI-M-kaken 2015-5p.pdf","filesize":[{"value":"386.8 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"PH-PR-WAKASUGI-M-kaken 2015-5p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/45334/files/PH-PR-WAKASUGI-M-kaken 2015-5p.pdf"},"version_id":"a684a6c3-1ee7-43ac-848d-7cb14e59c27d"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"休止期においてNER依存的に活性化するDNA損傷応答機構の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"休止期においてNER依存的に活性化するDNA損傷応答機構の解析"},{"subitem_title":"The study of the DNA damage response elicited by NER-provoked secondary damage in mammalian quiescent cells","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2819"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-07-13"},"publish_date":"2018-07-13","publish_status":"0","recid":"45334","relation_version_is_last":true,"title":["休止期においてNER依存的に活性化するDNA損傷応答機構の解析"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T09:37:54.630725+00:00"}