{"created":"2023-07-27T06:51:50.497530+00:00","id":45422,"links":{},"metadata":{"_buckets":{"deposit":"67fd50e8-bf08-42d9-983b-04d5d1955a1e"},"_deposit":{"created_by":18,"id":"45422","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"45422"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00045422","sets":["2812:2813:2817"]},"author_link":["78930","78929"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-05-26","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2013-04-01 - 2017-03-31","bibliographic_titles":[{"bibliographic_title":"平成28(2016)年度 科学研究費補助金 若手研究(A) 研究成果報告書"},{"bibliographic_title":"2016 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hanayama, Rikinari"}],"nameIdentifiers":[{"nameIdentifier":"78930","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40403191","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40403191"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"エクソソームは様々な細胞が放出する膜小胞で、分泌細胞と標的細胞の間で蛋白質やRNAなどを受け渡す媒体である。特に脳神経系においてエクソソームは種々の細胞から放出されるが、私達は神経細胞から放出されたエクソソームが、脳内の免疫細胞であるミクログリアに補体成分C3を発現させることにより、神経軸索・シナプスの剪定を促進することを見出した。また、エクソソームの膜表面に露出しているリン脂質ホスファチジルセリンと特異的に結合するTim4蛋白質を用いることで、高純度なエクソソームを再現性よく回収する方法の開発に成功し、従来の方法では同定できなかった数多くのエクソソーム上の蛋白質やRNAの同定を可能にした。","subitem_description_type":"Abstract"},{"subitem_description":"Exosomes are membrane vesicles released by various cells and are thought to deliver proteins, RNAs, etc. between secretory cells and target cells. In particular, exosomes are released from various cells in the cerebral nervous system (CNS), but we have found that exosomes released from neurons promote pruning of neuronal dendrites and synapses by inducing complement C3 in the target microglia, immune cells in CNS. In addition, we succeeded in developing a novel method for isolating highly purified exosomes with high reproducibility by using Tim4 protein that specifically binds to the phospholipid phosphatidylserine exposed on the surface of exosomes, which lead to the identification of many proteins and RNAs on exosomes that had never been identified by the conventional isolation methods.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:22790210, 研究期間(年度):2013-04-01 - 2017-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「パーキンソン病における神経系エクソソームの役割」研究成果報告書　課題番号22790210\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-25713020/25713020seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学新学術創成研究機構ナノ生命科学研究所","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00051759","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=40403191"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40403191","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25713020/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25713020/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-25713020/25713020seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-25713020/25713020seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"華山, 力成"}],"nameIdentifiers":[{"nameIdentifier":"78929","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40403191","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40403191"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-07-23"}],"displaytype":"detail","filename":"ME-PR-HANAYAMA-R-kaken 2017-4p.pdf","filesize":[{"value":"88.8 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-HANAYAMA-R-kaken 2017-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/45422/files/ME-PR-HANAYAMA-R-kaken 2017-4p.pdf"},"version_id":"0422fdf5-8432-4c14-9754-31f146cad60e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"パーキンソン病における神経系エクソソームの役割","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"パーキンソン病における神経系エクソソームの役割"},{"subitem_title":"Physiological functions of exosomes and indentification of their target cells","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2817"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-07-23"},"publish_date":"2018-07-23","publish_status":"0","recid":"45422","relation_version_is_last":true,"title":["パーキンソン病における神経系エクソソームの役割"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T05:39:21.736893+00:00"}