{"created":"2023-07-27T06:51:51.156756+00:00","id":45438,"links":{},"metadata":{"_buckets":{"deposit":"c3c717fa-6244-47e1-bd7d-02bca5c2a256"},"_deposit":{"created_by":18,"id":"45438","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"45438"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00045438","sets":["2812:2813:2817"]},"author_link":["78949","190"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-06-01","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2015-04-01 - 2017-03-31","bibliographic_titles":[{"bibliographic_title":"平成28(2016)年度 科学研究費補助金 挑戦的萌芽研究 研究成果報告書"},{"bibliographic_title":"2016 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shinmyo, Yohei"}],"nameIdentifiers":[{"nameIdentifier":"78949","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00418831","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00418831"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究では、子宮内エレクトロポレーションとCRISPR/Cas9システムとを組み合わせることにより、大脳皮質特異的な遺伝子ノックアウト法の確立を目指した。マウス大脳皮質にSatb2遺伝子に対するCRISPRコンストラクトを導入し、Satb2の発現を免疫染色により調べた。その結果、Satb2に対するCRISPRコンストラクトが導入された細胞のほとんどで、Satb2の発現が消失していた。これらの結果から、子宮内エレクトロポレーション法を用いたCRISPR/Cas9の導入により大脳皮質神経細胞において効果的に標的遺伝子をノックアウトできることが分かった。","subitem_description_type":"Abstract"},{"subitem_description":"The CRISPR/Cas9 system has recently been adapted for generating knockout mice to investigate physiological functions and pathological mechanisms. Here, we report a highly efficient procedure for brain-specific disruption of genes of interest in vivo. We constructed pX330 plasmids expressing humanized Cas9 and single-guide RNAs (sgRNAs) against the Satb2 gene, which encodes an AT-rich DNA-binding transcription factor. We found that the introduction of pX330-Satb2 into the developing mouse brain using in utero electroporation led to a dramatic reduction of Satb2 expression in the transfected cerebral cortex, suggesting DSBs had occurred in the Satb2 gene with high efficiency. Thus, our procedure combining the CRISPR/Cas9 system and in utero electroporation is an effective and rapid approach to achieve brain-specific gene knockout in vivo.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:15K14332, 研究期間(年度):2015-04-01 - 2017-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「大脳皮質特異的なノックアウト動物作成法の開発」研究成果報告書　課題番号15K14332\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K14332/15K14332seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00051775","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=00418831"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=00418831","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K14332/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K14332/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K14332/15K14332seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K14332/15K14332seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"新明, 洋平"}],"nameIdentifiers":[{"nameIdentifier":"190","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00418831","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00418831"},{"nameIdentifier":"00418831","nameIdentifierScheme":"金沢大学研究者情報","nameIdentifierURI":"http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=00418831"},{"nameIdentifier":"00418831","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000000418831"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-07-23"}],"displaytype":"detail","filename":"ME-PR-SHINMYO-Y-kaken 2017-4p.pdf","filesize":[{"value":"429.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-SHINMYO-Y-kaken 2017-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/45438/files/ME-PR-SHINMYO-Y-kaken 2017-4p.pdf"},"version_id":"280d2f92-2ddb-4028-a7c2-f4415efcd5a6"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"大脳皮質特異的なノックアウト動物作成法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"大脳皮質特異的なノックアウト動物作成法の開発"},{"subitem_title":"Gene knockout in the cerebral cortex","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2817"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-07-23"},"publish_date":"2018-07-23","publish_status":"0","recid":"45438","relation_version_is_last":true,"title":["大脳皮質特異的なノックアウト動物作成法の開発"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T05:38:08.260095+00:00"}