{"created":"2023-07-27T06:51:51.419843+00:00","id":45444,"links":{},"metadata":{"_buckets":{"deposit":"0eee1ad5-6087-469d-80f5-b20cb70818fe"},"_deposit":{"created_by":18,"id":"45444","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"45444"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00045444","sets":["2812:2813:2820"]},"author_link":["78956","78955"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-06-09","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5p.","bibliographicVolumeNumber":"2011-2013","bibliographic_titles":[{"bibliographic_title":"平成25(2013)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2013 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"(1)高転移性(H11)および低転移性(P29)ルイス肺がん細胞腫瘍内の血管の性質を解析した結果、高転移性腫瘍でLYVE1陽性のリンパ管内皮様細胞が認められ、DNAミクロアレイ解析でもLYVE1発現亢進が確認された。またNRP1の発現低下が示された。(2)H11およびP29細胞株から分離したRNAを用いたDNAミクロアレイ解析の結果、H11で発現上昇している遺伝子が約15種、発現低下している遺伝子が約10種同定された。(3)発現差が大きくかつ分泌性の蛋白質をコードする遺伝子を選択し、それらに対するshRNAを導入した細胞を作製してマウスに皮下移植し腫瘍内血管の構造を解析した。","subitem_description_type":"Abstract"},{"subitem_description":"The aim of this study was to clarify the molecular basis of the formation of vessels with different structures. (1) LYVE1-positive endothelial cells (ECs) were detected in tumors of high metastatic Lewis lung carcinoma cells (H11), but not in low metastatic cell (P29) tumors. (2) DNA microarray analyses using RNAs isolated from tumor vessel ECs demonstrated that LYVE1 expression was up-regulated in ECs in H11 tumor whereas NRP1 expression was down-regulated. These results suggested that H11 cells can induce lymphatic-like vessels in their tumors. (3) Expression profile analyses of H11 and P29 cells identified 15 up-regulated and 10 down-regulated genes in H11 cells. (4) We selected genes that encode secretory proteins and constructed lentiviral vectors expressing shRNAs against the candidate genes. We then prepared tumor cells expressing the shRNAs, transplanted the cells to mice, and analyzed the formation of tumors and their vessels.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:23590349, 研究期間(年度):2011-2013","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「高転移性ルイス肺癌細胞株が類洞様腫瘍血管を誘導する分子基盤の解明」課題番号23590349\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23590349/23590349seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00051781","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学医薬保健研究域医学系 / 金沢医科大学"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=80240373"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=80240373","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590349/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590349/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23590349/23590349seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23590349/23590349seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-07-23"}],"displaytype":"detail","filename":"ME-PR-YONEKURA-H-kaken 2014-5p.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-YONEKURA-H-kaken 2014-5p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/45444/files/ME-PR-YONEKURA-H-kaken 2014-5p.pdf"},"version_id":"973ace9f-4647-4bd8-9b4d-ad7c0b6b5bc2"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"高転移性ルイス肺癌細胞株が類洞様腫瘍血管を誘導する分子基盤の解明","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"高転移性ルイス肺癌細胞株が類洞様腫瘍血管を誘導する分子基盤の解明"},{"subitem_title":"Molecular basis of the formation of blood vessels with different structures","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2820"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-07-23"},"publish_date":"2018-07-23","publish_status":"0","recid":"45444","relation_version_is_last":true,"title":["高転移性ルイス肺癌細胞株が類洞様腫瘍血管を誘導する分子基盤の解明"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T09:18:18.965842+00:00"}