{"created":"2023-07-27T06:52:07.285817+00:00","id":45866,"links":{},"metadata":{"_buckets":{"deposit":"b91b2989-390e-4118-b1a3-281b12f71ad5"},"_deposit":{"created_by":18,"id":"45866","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"45866"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00045866","sets":["2812:2813:2820"]},"author_link":["163","79691"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-06-02","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5p.","bibliographicVolumeNumber":"2011-2013","bibliographic_titles":[{"bibliographic_title":"平成25(2013)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2013 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"バイオ燃料電池の実用化に必須である白金代替酸素還元触媒の創成を目的として，酸素の４電子還元反応を触媒するマルチ銅オキシダーゼをターゲットに，高反応性酵素の探索と既存酵素のタンパク質工学的高機能化を行った。探索研究では，嫌気性細菌のラッカーゼと，昆虫のクチクラ硬化ラッカーゼ遺伝子の発現系を構築し，前者が既存酵素と全く異なる性質を持つ事を明らかにした。さらに，微生物由来の既存酵素をベースとしたタンパク質工学的改変により，酸素還元活性，電極反応性の向上に成功した。","subitem_description_type":"Abstract"},{"subitem_description":"For the development of the platinum-substitute anodic enzyme catalysts for bio-fuel cell, I performed the in silico screening to find new multi-copper oxidases and the mutations against the common enzymes to improve their oxygen reduction activity. In the screening for the new multi-copper oxidases, I found two laccase genes from an anaerobic bacterium and an insect. The heterologous expression systems for the laccase genes were successfully constructed, and the recombinant laccase from anaerobe was found to have unreported properties so far. Furthermore, the improvements of the bacterial common enzymes in the oxygen-reduction activity and the electrode response were achieved by protein engineering.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:23580131, 研究期間(年度):2011-2013","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「バイオ燃料電池の実用化に必須である高反応性カソード電極用酵素の研究開発」課題番号23580131\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23580131/23580131seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00052200","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学理工研究域物質化学系"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=40252712"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40252712","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580131/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580131/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23580131/23580131seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23580131/23580131seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-09-28"}],"displaytype":"detail","filename":"SC-PR-KATAOKA-K-kaken 2014-5p.pdf","filesize":[{"value":"258.1 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC-PR-KATAOKA-K-kaken 2014-5p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/45866/files/SC-PR-KATAOKA-K-kaken 2014-5p.pdf"},"version_id":"85e48667-32d6-4d6e-b6ad-244f39802391"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"バイオ燃料電池の実用化に必須である高反応性カソード電極用酵素の研究開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"バイオ燃料電池の実用化に必須である高反応性カソード電極用酵素の研究開発"},{"subitem_title":"Research and development of the highly-active cathodic enzyme necessary for the practical use of the bio-fuel cell","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2820"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-10-01"},"publish_date":"2018-10-01","publish_status":"0","recid":"45866","relation_version_is_last":true,"title":["バイオ燃料電池の実用化に必須である高反応性カソード電極用酵素の研究開発"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T09:16:36.923637+00:00"}