{"created":"2023-07-27T06:52:22.617889+00:00","id":46259,"links":{},"metadata":{"_buckets":{"deposit":"03e31625-302d-41ba-be6e-60da3341375c"},"_deposit":{"created_by":18,"id":"46259","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"46259"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00046259","sets":["2812:2813:2816"]},"author_link":["80160","80159"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-05-25","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5p.","bibliographicVolumeNumber":"2015-04-01 - 2018-03-31","bibliographic_titles":[{"bibliographic_title":"平成29(2017)年度 科学研究費補助金 挑戦的萌芽研究 研究成果報告書"},{"bibliographic_title":"2017 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Endoh, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"80160","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20272932","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20272932"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"テトラヒメナを用いて外来遺伝子の発現ベクターの開発・改良を行った。このベクターは、１）耐性遺伝子の入ったスクリーニング用カセット、２）システインプロテアーゼの分泌シグナルを組み込んだ発現カセット、３）相同組換えのための導入遺伝子座の周辺配列、からなる。このベクターに、シロアリ腸内共生鞭毛虫由来の４種のセルラーゼ遺伝子の発現誘導・分泌後、セルラーゼ活性を測定した。新ベクターでは、以前のベクターに比べ、EG5、EG7両方で10～50倍程度の活性が検出され。リグニンエステラーゼであるCip2-likeに関して、若干の作用が認められた。CBHについては、これまでのところ活性は検出できていない。","subitem_description_type":"Abstract"},{"subitem_description":"Construction of a novel transformation vector for expressing and secreting foreign cellulase genes in the ciliate Tetrahymena thermopile. This vector consists of 3 separate parts: 1) a cassette carrying drug resistant gene for screening after bombardment, 2) an expression cassette with a secretion signal derived from Tetrahymena cystein protease 4 gene, 3) flanking sequences at both sides of beta-tubulin gene for homologous recombination. Introducing 4 different genes (endoglucanase (EG) 5 and 7, cellobiohydrolase (CBH) and lignin esterase Cip2-like) from the termite symbiotic protists for cellulolysis into the vector, their activities were measured after induction of gene expression and subsequent secretion outside cells, respectively. EG5 and 7 were efficiently expressed, showing strong activity. Lignin esterase Cip2-like activity was slightly detected. No CBH activity has been confirmed, suggesting difficult folding due to the complicated protein structure.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:15K12263, 研究期間(年度):2015-04-01 - 2018-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「原生生物テトラヒメナによる新規セルロース分解系の構築」課題番号15K12263\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K12263/15K12263seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学理工研究域生命理工学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00052592","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=20272932"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=20272932","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K12263/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K12263/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K12263/15K12263seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K12263/15K12263seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"遠藤, 浩"}],"nameIdentifiers":[{"nameIdentifier":"80159","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20272932","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20272932"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-04-26"}],"displaytype":"detail","filename":"SC-PR-ENDOH-H-kaken 2018-5p.pdf","filesize":[{"value":"175.8 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC-PR-ENDOH-H-kaken 2018-5p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/46259/files/SC-PR-ENDOH-H-kaken 2018-5p.pdf"},"version_id":"2c4696b2-ba83-4cdc-a23e-354ec95f08f1"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"原生生物テトラヒメナによる新規セルロース分解系の構築","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"原生生物テトラヒメナによる新規セルロース分解系の構築"},{"subitem_title":"Construction of a novel celluloysis system utilizing Tetrahymena","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2816"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-04-26"},"publish_date":"2019-04-26","publish_status":"0","recid":"46259","relation_version_is_last":true,"title":["原生生物テトラヒメナによる新規セルロース分解系の構築"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T05:35:48.283122+00:00"}