{"created":"2023-07-27T06:52:30.911706+00:00","id":46485,"links":{},"metadata":{"_buckets":{"deposit":"af1ae216-7fb2-4476-ae15-7a9c5284bfe1"},"_deposit":{"created_by":18,"id":"46485","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"46485"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00046485","sets":["2812:2813:2817"]},"author_link":["2361","71374"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-05-04","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"6p.","bibliographicVolumeNumber":"2012-04-01 - 2017-03-31","bibliographic_titles":[{"bibliographic_title":"平成28(2016)年度 科学研究費補助金 基盤研究(S) 研究成果報告書"},{"bibliographic_title":"2016 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"三つの課題に取り組んだ。課題1では、既に確立した高速AFMを利用して多様な蛋白質系で起こる動的プロセスを観察し、機能メカニズムに迫るとともに、従来技術では困難な天然変性蛋白質の構造解析が高速AFMで可能であることを実証した。課題2では、振動を起こさずに広域を高速走査する技術やイメージング中に試料を操作可能なインターラクティブ高速AFMを開発し、その有効性を実証した。また、カンチレバー走査方式の高速AFMと蛍光顕微鏡との複合機を開発し、蛍光像と高速AFM像の同時取得を実現した。課題3では、非接触観察可能な走査型イオン伝導顕微鏡の高速化に向け要素技術を開発し、約100倍の高速化に成功した。","subitem_description_type":"Abstract"},{"subitem_description":"We worked on three subjects. In subject 1, we elucidated the functional mechanism of various protein systems by observing their dynamic processes using high-speed AFM (HS-AFM) that we had established before. Besides, we demonstrated that HS-AFM can analyze the structure of intrinsically disordered proteins that are difficult to analyze with conventional methods. In subject 2, we developed fast/wide-area scanning techniques without generation of unwanted vibrations and an interactive HS-AFM technique that can manipulate the sample during imaging. The usefulness of these new techniques were demonstrated. In addition, we developed cantilever-scan type of HS-AFM combined with fluorescence microscopy, and thereby, made it possible to capture HS-AFM and fluorescence images simultaneously. In subject 3, we developed high-speed scanning ion conductance microscopy that can image the sample without contact with the sample. The imaging speed reached a level 100-times faster than before.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:24227005, 研究期間(年度):2012-04-01 - 2017-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「高速バイオAFMが拓く新構造生物学」研究成果報告書　課題番号24227005\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24227005/24227005seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学新学術創成研究機構ナノ生命科学研究所 ","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00052817","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=50184320"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=50184320","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24227005/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24227005/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24227005/24227005seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24227005/24227005seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-19"}],"displaytype":"detail","filename":"SC-PR-ANDO-T-kaken 2017-6p.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC-PR-ANDO-T-kaken 2017-6p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/46485/files/SC-PR-ANDO-T-kaken 2017-6p.pdf"},"version_id":"f10d441e-2282-47b0-90c5-1ee496dfeada"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"高速バイオAFMが拓く新構造生物学","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"高速バイオAFMが拓く新構造生物学"},{"subitem_title":"Opening up New Structural Biology by High-speed AFM","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2817"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-11-19"},"publish_date":"2018-11-19","publish_status":"0","recid":"46485","relation_version_is_last":true,"title":["高速バイオAFMが拓く新構造生物学"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T11:08:32.232327+00:00"}