{"created":"2023-07-27T06:52:31.177007+00:00","id":46492,"links":{},"metadata":{"_buckets":{"deposit":"87441156-1056-47a1-9397-16fef855c4ca"},"_deposit":{"created_by":18,"id":"46492","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"46492"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00046492","sets":["2812:2813:2820"]},"author_link":["80523","2506"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-06-02","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"6p.","bibliographicVolumeNumber":"2011-2013","bibliographic_titles":[{"bibliographic_title":"平成25(2013)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2013 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"葉緑体型ATP合成酵素の部分複合体であるα3β3γに存在する挿入配列とε阻害の関係を構造生物学的側面から理解するために、α3β3γεのX線結晶構造解析に取り組んだ。これまで、結晶化に適した変異α3β3γεの作成に成功し、結晶を得ることができたが、高分解能のデータはまだ得られていない。\nまた、葉緑体型ATP合成酵素の活性制御に重要な挿入配列がどのように遠く離れた触媒部位であるβサブユニットに影響しているかを生化学的手法により検証し、γサブユニット上のN末端およびC末端のα-ヘリックスのコイルドコイル構造の相対的なズレが、挿入配列による活性制御効果をβサブユニットに伝達している可能性が示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"Inserted region on the gamma subunit of chloroplast type ATP synthase strongly affect to the inhibitory effect of endogenous epsilon subunit (epsilon inhibition). To understand the relationship between the inserted region and the epsilon subunit, we try to dissolve structure of sub-complex of chloroplast type ATP synthase (alpha3beta3gamma1epsilon1). Though we could not yet get crystal in high resolution, we succeeded to make stable sub-complex mutant for crystallization and could get some crystal.\nWe also investigated an affect of the inserted region on the gamma subunit to the ATPase activity on the beta subunit because these two parts exist far form each other. We then identified a conformational change of two central alpha helices in gamma subunit which is interact with catalytic sites on the beta subunit is necessary to read regulatory effect of the inserted region.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:23570159, 研究期間(年度):2011-2013","subitem_description_type":"Other"},{"subitem_description":"出典：「葉緑体型ATP合成酵素の活性調節機構を構造の側面から理解する」研究成果報告書　課題番号23570159\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23570159/23570159seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00052824","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学新学術創成研究機構ナノ生命科学研究所"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=80419267"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=80419267","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570159/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570159/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23570159/23570159seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23570159/23570159seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-19"}],"displaytype":"detail","filename":"SC-PR-KONNO-H-kaken 2014-6p.pdf","filesize":[{"value":"584.4 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC-PR-KONNO-H-kaken 2014-6p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/46492/files/SC-PR-KONNO-H-kaken 2014-6p.pdf"},"version_id":"e5290af7-1bb6-4518-ad38-c5ea5f4f0665"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"葉緑体型ATP合成酵素の活性調節機構を構造の側面から理解する","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"葉緑体型ATP合成酵素の活性調節機構を構造の側面から理解する"},{"subitem_title":"Understanding of the regulatory mechanism on the activity of chloroplast-type ATP synthase by X-ray crystallography","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2820"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-11-19"},"publish_date":"2018-11-19","publish_status":"0","recid":"46492","relation_version_is_last":true,"title":["葉緑体型ATP合成酵素の活性調節機構を構造の側面から理解する"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T09:10:46.233803+00:00"}