{"created":"2023-07-27T06:52:31.265258+00:00","id":46495,"links":{},"metadata":{"_buckets":{"deposit":"2c089ce7-6701-4c21-b6c4-01b63b78ba45"},"_deposit":{"created_by":18,"id":"46495","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"46495"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00046495","sets":["2812:2813:2816"]},"author_link":["2505","80527"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-05-21","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2015-04-01 - 2018-03-31","bibliographic_titles":[{"bibliographic_title":"平成29(2017)年度 科学研究費補助金 挑戦的萌芽研究 研究成果報告書"},{"bibliographic_title":"2017 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"昆虫の後胚発生において、脱皮は体サイズの成長に重要な現象である。この脱皮のタイミングは前胸腺で合成・分泌されるエクジソンによって制御され，さらにエクジソンの合成は脳のPTTH(前胸腺刺激ホルモン)細胞によって制御されている。本研究ではPTTH細胞の神経活動をカルシウムイメージングによって詳細に調べ，発生時における生理学的機能を明らかにした。またPTTH細胞の神経活動の人為的操作法やRNA-Seq解析法を確立した。さらに，PTTH遺伝子ノックアウト系統を作出し，発生におけるPTTHの重要性を解析した。","subitem_description_type":"Abstract"},{"subitem_description":"In the postembryonic development of insects, ecdysis is an important event for the animals to increase their body size. Timing of ecdysis is thus tightly controlled by the molting hormone ecdysone synthesized in the prothoracic glands, whose activity is regulated by prothoracicotropic hormone (PTTH) neurons in the brain. In the present study, we investigated neural activity pattern of PTTH neurons during development by G-GaCMP6f-mediated Ca imaging and revealed their physiological function. We also established silkmoth strains that can artificially manipulate level of neural activity by chemogenetic or optogenetic approaches. Furthermore, we established a PTTH-gene-knockout line and analyzed importance of PTTH on insect development.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:15K14895, 研究期間(年度):2015-04-01 - 2018-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「シングルセル内分泌学的手法による昆虫変態マスター細胞の検証」課題番号15K14895\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K14895/15K14895seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学理工研究域生命理工学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00052827","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=40193768"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40193768","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K14895/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K14895/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K14895/15K14895seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K14895/15K14895seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-04-26"}],"displaytype":"detail","filename":"EC-PR-IWAMI-M-kaken 2018-4p.pdf","filesize":[{"value":"454.8 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"EC-PR-IWAMI-M-kaken 2018-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/46495/files/EC-PR-IWAMI-M-kaken 2018-4p.pdf"},"version_id":"80450887-9d75-420d-89c0-80977f252107"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"シングルセル内分泌学的手法による昆虫変態マスター細胞の検証","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"シングルセル内分泌学的手法による昆虫変態マスター細胞の検証"},{"subitem_title":"Verification of insect metamorphosis master cells by single cell endocrinology approach","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2816"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-04-26"},"publish_date":"2019-04-26","publish_status":"0","recid":"46495","relation_version_is_last":true,"title":["シングルセル内分泌学的手法による昆虫変態マスター細胞の検証"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:47:21.734805+00:00"}