{"created":"2023-07-27T06:52:36.622578+00:00","id":46639,"links":{},"metadata":{"_buckets":{"deposit":"ff74bc95-dc64-42bc-97ff-dfcff318e466"},"_deposit":{"created_by":18,"id":"46639","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"46639"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00046639","sets":["2812:2813:2817"]},"author_link":["81163","81164"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-05-24","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2014-04-01 - 2017-03-31","bibliographic_titles":[{"bibliographic_title":"平成28(2016)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2016 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"マウス骨髄細胞からpsolaren と紫外線(UVA)を用いて、制御性樹状細胞(PUVA-DC)を作製する技術を開発した。In vitroの解析ではMHC 非依存性にT リンパ球に対し抑制作用を示し、細胞間接着およびIDO増加による免疫寛容誘導が示唆された。しかしIDO阻害物質下におけるリンパ球混合培養では免疫抑制作用は解除されなかった。マウスGVHDモデルでPUVA-DC（Host,Donor,Third-party）を輸注したがGVHD抑制作用は認めなかった。生体内での生着・生存が不十分であることが考えられ、生体内投与後においても生存するPUVA-DC作成を探索する研究へと繋がった。","subitem_description_type":"Abstract"},{"subitem_description":"Bone marrow-derived cultured DCs acquired tolerogenicity by PUVA (psoralen+UVA) treatment in mice. In vitro assays, strong tolerogenicity was observed when adding PUVA-DC even from third party strain into MLR. That is, the PUVA-DC have tolerogenic function in a MHC-independent manner. We showed that cell-to-cell contact was needed to mediate the regulatory effect by transwell experiment. Next, we compared the expression of the IDO, which induces T-cell anergy, by real-time PCR between PUVA-DC and BM-DC. An increase IDO gene transcription level was observed in PUVA-DC about 5 times more than in BM-DC. But adding 1-MT, which is an inhibitor of IDO, had no effects on MLR with PUVA-DC.\nWe transfused PUVA-DC (Host-type, Donor-type, Third-party-type) in a mouse GVHD model, but the GVHD inhibitory effects were not observed. It was thought the survival of PUVA-DC was insufficient. These data leads us to search for technique to make PUVA-DC to survive after in vivo administration.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:26461604, 研究期間(年度):2016-04-01 - 2018-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「PUVA処理した制御性樹状細胞によるヒトGVHDに対する新たな細胞療法の開発」研究成果報告書　課題番号26461604\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26461604/26461604seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学附属病院小児科","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00052971","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=10419335"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=10419335","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26461604/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26461604/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26461604/26461604seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26461604/26461604seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-12-21"}],"displaytype":"detail","filename":"ME-PR-MAEBA-H-kaken 2017-4p.pdf","filesize":[{"value":"288.1 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-MAEBA-H-kaken 2017-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/46639/files/ME-PR-MAEBA-H-kaken 2017-4p.pdf"},"version_id":"d8f2a7ac-4dfb-4ef9-91e4-d2f6aa259629"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"PUVA処理した制御性樹状細胞によるヒトGVHDに対する新たな細胞療法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"PUVA処理した制御性樹状細胞によるヒトGVHDに対する新たな細胞療法の開発"},{"subitem_title":"Development of the new cell therapy for GVHD using tolerogenic dendtiric cells treated by PUVA treatment","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2817"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-12-21"},"publish_date":"2018-12-21","publish_status":"0","recid":"46639","relation_version_is_last":true,"title":["PUVA処理した制御性樹状細胞によるヒトGVHDに対する新たな細胞療法の開発"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T11:08:22.438011+00:00"}