@article{oai:kanazawa-u.repo.nii.ac.jp:00046709,
 author = {御影, 雅幸 and Han, Yan and Kitaoka, Fumiyo and Mano, Masumi and Sasaki, Yohei and Mikage, Masayuki},
 issue = {3},
 journal = {Journal of Traditional Medicines},
 month = {},
 note = {漢方生薬 「威霊仙」 はキンポウゲ科のセンニンソウ属植物の地下部に由来する。 近年の生薬市場には多数の原植物に由来する威霊仙が見られるが， 日・中の薬局方では原植物を Clematis hexapetala, C. mandshurica および C. chinensisの 3 種であると規定している。 現在日本， 中国及び韓国市場の威霊仙の原植物を明らかにする目的で， 東アジアに産するセンニンソウ属植物のうち関連する 9 分類群について， 分子生物学的に ITS 領域を検討した。 その結果， ITS 全領域の塩基配列を検討することにより， 9 分類群を区別することができた。 以上の結果から， 現在市場で入手した11サンプルの威霊仙を分子遺伝学的に同定することができた。 すなわち， 日本市場品は 4 サンプルのうち 3 つは C. mandshurica で， 1 つは C. chinensis であった。 中国市場品 4 サンプルのうち 2 つは C. mandshurica で， 他の 2 つは C. brachyura であった。 韓国産 3 サンプルのうち 2 つは C. mandshurica で， 他の 1 つは C. terniflora var. robusta であった。, Clematidis Radix (Wei Ling Xian in Chinese and Ireisen in Japanese) is a crude drug used in traditional Chinese medicine and is derived from the underground parts of Clematis plants, which belong to the family Ranunculaceae. The Clematidis Radix sold in modern markets is derived from a variety of botanical origins, whereas the Chinese and Japanese pharmacopoeias state that Clematidis Radix should be produced from Clematis hexapetala, C. mandshurica, or C. chinensis. To clarify the botanical origin of this crude drug, 9 closely related taxa of the genus Clematis growing in East Asia were subjected to molecular biological studies of their internal transcribed spacer (ITS) regions. We found that the ITS region nucleotide sequences of the 9 taxa had diverged. As a result, the 9 taxa could be successfully differentiated by comparing their whole ITS region sequences. Based on this result, 11 Clematidis Radix samples obtained from modern Japanese, Chinese, and Korean markets were genetically identified as follows: Of the samples from Japanese markets, 3 out of 4 were categorized as C. mandshurica, and one as C. chinensis. Among the samples from Chinese markets, 2 out of 4 were identified as C. mandshurica, and the other two as C. brachyura. Meanwhile, of the 3 samples from Korean markets, two were identified as C. mandshurica, and the other as C. terniflora var. robusta. © 2013, Medical and Pharmaceutical Society for WAKAN-YAKU. All rights reserved., 金沢大学医薬保健研究域薬学系},
 pages = {91--101},
 title = {ITS analysis of Clematis plants from East Asia and the botanical origin of Clematidis Radix sold in modern markets},
 volume = {30},
 year = {2013}
}