@article{oai:kanazawa-u.repo.nii.ac.jp:00046711,
 author = {堂井, 美里 and 垣内, 信子 and 御影, 雅幸 and Shi, Wen and Kitaoka, Fumiyo and Doui, Misato and Miyake, Katsunori and Sasaki, Yohei and Kakiuchi, Nobuko and Mikage, Masayuki},
 issue = {2},
 journal = {Journal of Traditional Medicines},
 month = {},
 note = {日本薬局方ではアカネ科 Uncaria sinensis Havil., U. rhynchophylla Miq. 及び U. macrophylla Wall. の鉤を釣藤鉤として規定している。 釣藤鉤には異なる薬効を示すインドール及びオキシインドールアルカロイドが含まれているが， これらの組成は原植物の違いにより特徴的に異なるとされる。 しかし， Uncaria 属植物のアルカロイド組成に影響を与える要因については報告されていない。 本研究では， 広域で採集した Uncaria 属植物の種を DNA 塩基配列により正確に同定した後に， アルカロイド （rhynchophylline, geissoschizine methyl ether 及び hirsutine） 含量を HPLC 法により測定した。 その結果， 降水量が低い地域に生育する U. rhynchophyllaの鉤及び茎にアルカロイド含量が高い傾向が認められた。 また， 同一種であっても， 栽培品と野生品のアルカロイド組成は異なり， 市場品 「釣藤鉤」 には rhynchophylline 優位のタイプと geissoschizine methyl ether と hirsutine 優位のタイプの 2 種が確認された。 加えて， 釣藤鉤の原植物を正確に同定するために， DNA 塩基配列解析及び PCR-RFLP 法を用いた分子系統学的手法を確立した。, The Japanese Pharmacopoeia states that the crude drug Chotoko is composed of the hooks of Uncaria rhynchophylla Miq., U. sinensis Havil., and U. macrophylla Wall., all of which belong to the Rubiaceae family. It has been reported that the indole and oxindole type alkaloids contained in Chotoko have different pharmacological effects, and Chotoko products derived from different species are composed of different alkaloids. However, there are no reports about the factors affecting the chemical compositions of Uncaria plants. In this study, we analyzed the alkaloid contents (the rhynchophylline, geissoschizine methyl ether, and hirsutine contents) of Uncaria samples collected from a broad range of sites by HPLC after identifying their species by DNA sequence analysis. As a result, we found that the hooks and small stems of U. rhynchophylla grown in habitats with lower annual precipitation levels tended to display higher alkaloid contents. We also found that the alkaloid compositions of cultivated Uncaria plants were different from those of wild plants, even those belonging to the same species, and crude Chotoko products displayed two types of alkaloid profile, the rhynchophylline-rich type and the geissoschizine methyl ether and hirsutine-rich type. Moreover, in order to accurately identify the botanical origin of Chotoko, we established a method involving molecular genetics techniques; i.e., DNA sequence analysis and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). © 2012, Medical and Pharmaceutical Society for WAKAN-YAKU. All rights reserved., 金沢大学医薬保健研究域薬学系},
 pages = {63--73},
 title = {Quality evaluation of Chotoko-Local and specific variations in the alkaloid contents of Uncaria plants},
 volume = {29},
 year = {2012}
}