@article{oai:kanazawa-u.repo.nii.ac.jp:00047256, author = {Enkawa, Chisato and Aizawa, Mineaki and Nakayama, Chisato and Iizuka, Kazuya and Ohkubo, Tatsuhiro and 遠川, 千聡 and 逢沢, 峰昭 and 中山, ちさ and 飯塚, 和也 and 大久保, 達}, issue = {1}, journal = {植物地理・分類研究, The journal of phytogeography and taxonomy}, month = {Sep}, note = {This study aimed to clarify clonal structure and reproductive modes, layering (asexual) and/or seedling regeneration (sexual), of Quercus crispula var. horikawae using eight nuclear EST-SSR markers; the species has stunt creeping multiple stems and occurs in pseudo-alpine zone of the mountains in the Sea of Japan side. This study was conducted in an isolated stand (alt. 1,570 m) of the species near Ohtoge-pass in Mts. Nasu, northern Tochigi Prefecture. DNA was extracted from leaves of 92 stems that did not have any distinct connection of roots and creeping stems above the ground. The results of DNA analysis indicated that the stand consisted of four genets (clones). Genet A, which had 87 stems (ramets), occupied throughout the study plot. The other clones (B, C and D), which were composed of 1-3 ramets, might be derived from seedling regeneration. In conclusion, reproductive modes in the stand were likely to be dominated by layering regeneration resulted from the creeping of stems rather than seedling regeneration that rarely occurred.}, pages = {1--8}, title = {那須山系におけるミヤマナラの更新様式}, volume = {63}, year = {2015} }