@article{oai:kanazawa-u.repo.nii.ac.jp:00047486, author = {喜多村, 晃一 and 村松, 正道 and Watashi, Koichi and Liang, Guoxin and Iwamoto, Masashi and Marusawa, Hiroyuki and Uchida, Nanako and Daito, Takuji and Kitamura, Kouichi and Muramatsu, Masamichi and Ohashi, Hirofumi and Kiyohara, Tomoko and Suzuki, Ryosuke and Li, Jisu and Tong, Shuping and Tanaka, Yasuhito and Murata, Kazumoto and Aizaki, Hideki and Wakita, Takaji}, issue = {44}, journal = {Journal of Biological Chemistry}, month = {Nov}, note = {Virus infection is restricted by intracellular immune responses in host cells, and this is typically modulated by stimulation of cytokines. The cytokines and host factors that determine the host cell restriction against hepatitis B virus (HBV) infection are not well understood. We screened 36 cytokines and chemokines to determine which were able to reduce the susceptibility of HepaRG cells to HBV infection. Here, we found that pretreatment with IL-1β and TNFα remarkably reduced the host cell susceptibility to HBV infection. This effect was mediated by activation of the NF-κB signaling pathway. A cytidine deaminase, activation-induced cytidine deaminase (AID), was up-regulated by both IL-1β and TNFα in a variety of hepatocyte cell lines and primary human hepatocytes. Another deaminase APOBEC3G was not induced by these proinflammatory cytokines. Knockdown of AID expression impaired the anti-HBV effect of IL-1β, and overexpression of AID antagonized HBV infection, suggesting that AID was one of the responsible factors for the anti-HBV activity of IL-1/TNFα. Although AID induced hypermutation of HBV DNA, this activity was dispensable for the anti-HBV activity. The antiviral effect of IL-1/TNFα was also observed on different HBV genotypes but not on hepatitis C virus. These results demonstrate that proinflammatory cytokines IL-1/TNFα trigger a novel antiviral mechanism involving AID to regulate host cell permissiveness to HBV infection., 金沢大学医薬保健研究域医学系}, pages = {31715--31727}, title = {Interleukin-1 and tumor necrosis factor-α trigger restriction of hepatitis B virus infection via a cytidine deaminase activation-induced cytidine deaminase (AID).}, volume = {288}, year = {2013} }