{"created":"2023-07-27T06:53:11.363060+00:00","id":47545,"links":{},"metadata":{"_buckets":{"deposit":"ffb3fde4-70ad-4581-8611-27545d9f8d47"},"_deposit":{"created_by":18,"id":"47545","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"47545"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00047545","sets":["4278:2147:2148"]},"author_link":["84775","84772","84774","84773"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"142","bibliographicPageStart":"137","bibliographicVolumeNumber":"65","bibliographic_titles":[{"bibliographic_title":"BioTechniques"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"滝野, 隆久"}],"nameIdentifiers":[{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A modified invasion assay using a three-dimensional collagen gel was developed that enables isolation of invasive living cells; it was named the invading cell trapping (iCT) assay. A small cell strainer consisting of a nylon mesh with 40-μm2 pores was used, and collagen gel layers formed across the membrane. Test cells were seeded in the lower gel layer and invasive cells were attracted upward and trapped in the upper gel. After incubation, the collagen gel layers in cell strainers were easily separated and living cells in the gel were counted and analyzed. An advantage of the iCT assay is that it can capture living invasive cells in the upper gel while leaving noninvasive ones in the lower layer. Further enrichment of the two cell populations can be achieved by repeating the assay. Thus, the iCT assay allows comparative analysis of invasive versus noninvasive cells.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学国際基幹教育院GS教育系 / Institute of Liberal Arts and Science","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00053869","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Future Medicine"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.2144/btn-2018-0036","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.future-science.com/doi/10.2144/btn-2018-0036"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.future-science.com/doi/10.2144/btn-2018-0036","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"copyright © "}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA1066297X","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0736-6205","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takino, Takahisa"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Sato, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Seiki, Motoharu"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-04-12"}],"displaytype":"detail","filename":"GS-PR-TAKINO-T-0036.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"GS-PR-TAKINO-T-0036.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/47545/files/GS-PR-TAKINO-T-0036.pdf"},"version_id":"4eea60bf-d830-4ae8-b459-065efdb002ce"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Simple and cost-effective assay for isolating invasive living cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Simple and cost-effective assay for isolating invasive living cells"}]},"item_type_id":"4","owner":"18","path":["2148"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-04-12"},"publish_date":"2019-04-12","publish_status":"0","recid":"47545","relation_version_is_last":true,"title":["Simple and cost-effective assay for isolating invasive living cells"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T16:33:14.768378+00:00"}