{"created":"2023-07-27T06:53:11.988510+00:00","id":47559,"links":{},"metadata":{"_buckets":{"deposit":"2eb3baf4-5285-4809-8134-18b8ab8ccd5d"},"_deposit":{"created_by":18,"id":"47559","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"47559"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00047559","sets":["1132:1133:1134"]},"author_link":["23919","80335","23442","707","84815","21404","174","84833","55","76763"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"280","bibliographicPageStart":"263","bibliographicVolumeNumber":"69","bibliographic_titles":[{"bibliographic_title":"Journal of Physiological Sciences"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"吉岡, 和晃"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"安藝, 翔"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"多久和, 典子"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"多久和, 陽"}],"nameIdentifiers":[{},{},{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Pinocytosis is an important fundamental cellular process that is used by the cell to transport fluid and solutes. Phosphoinositide 3-kinases (PI3Ks) regulate a diverse array of dynamic membrane events. However, it is not well-understood which PI3K isoforms are involved in specific mechanisms of pinocytosis. We performed knockdown studies of endogenous PI3K isoforms and clathrin heavy chain (CHC) mediated by small interfering RNA (siRNA). The results demonstrated that the class II PI3K PI3K-C2α and PI3K-C2β, but not the class I or III PI3K, were required for pinocytosis, based on an evaluation of fluorescein-5-isothiocyanate (FITC)–dextran uptake in endothelial cells. Pinocytosis was partially dependent on both clathrin and dynamin, and both PI3K-C2α and PI3K-C2β were required for clathrin-mediated—but not clathrin-non-mediated—FITC-dextran uptake at the step leading up to its delivery to early endosomes. Both PI3K-C2α and PI3K-C2β were co-localized with clathrin-coated pits and vesicles. However, PI3K-C2β, but not PI3K-C2α, was highly co-localized with actin filament-associated clathrin-coated structures and required for actin filament formation at the clathrin-coated structures. These results indicate that PI3K-C2α and PI3K-C2β play differential, indispensable roles in clathrin-mediated pinocytosis. © 2018, The Physiological Society of Japan and Springer Japan KK, part of Springer Nature.","subitem_description_type":"Abstract"}]},"item_4_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Embargo Period 12 months","subitem_description_type":"Other"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00053883","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Physiological Society of Japan 日本生理学会 / Springer Nature"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s12576-018-0644-2","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://link.springer.com/article/10.1007%2Fs12576-018-0644-2"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://link.springer.com/article/10.1007%2Fs12576-018-0644-2","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"copyright © 2018, The Physiological Society of Japan and Springer Japan KK, part of Springer Nature."}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA12129145","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1880-6546","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Aung, Khin Thuzar"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshioka, Kazuaki"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Aki, Sho"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Ishimaru, Kazuhiro"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Takuwa, Noriko"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Takuwa, Yoh"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-02"}],"displaytype":"detail","filename":"ME-PR-TAKUWA-Y-263.pdf","filesize":[{"value":"10.1 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-TAKUWA-Y-263.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/47559/files/ME-PR-TAKUWA-Y-263.pdf"},"version_id":"3d4d2fa5-4fb2-4464-aa0a-950e5db1b2e8"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-02"}],"displaytype":"detail","filename":"ME-PR-TAKUWA-Y-263-supplementary video 1.mp4","filesize":[{"value":"2.5 MB"}],"format":"video/mp4","licensetype":"license_11","mimetype":"video/mp4","url":{"label":"ME-PR-TAKUWA-Y-263-supplementary video 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differentially regulate clathrin-dependent pinocytosis in human vascular endothelial cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The class II phosphoinositide 3-kinases PI3K-C2α and PI3K-C2β differentially regulate clathrin-dependent pinocytosis in human vascular endothelial cells"}]},"item_type_id":"4","owner":"18","path":["1134"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-04-25"},"publish_date":"2019-04-25","publish_status":"0","recid":"47559","relation_version_is_last":true,"title":["The class II phosphoinositide 3-kinases PI3K-C2α and PI3K-C2β differentially regulate clathrin-dependent pinocytosis in human vascular endothelial cells"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T16:32:28.339156+00:00"}