{"created":"2023-07-27T06:53:15.793612+00:00","id":47657,"links":{},"metadata":{"_buckets":{"deposit":"d3901281-3124-45ce-b6bd-7b709e6bc776"},"_deposit":{"created_by":18,"id":"47657","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"47657"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00047657","sets":["2812:2813:2815"]},"author_link":["69318","80133"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-06-10","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2016-04-01 - 2019-03-31","bibliographic_titles":[{"bibliographic_title":"平成30(2018)年度 科学研究費補助金 基盤研究(B) 研究成果報告書"},{"bibliographic_title":"2018 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究では，「リアルタイム」で「特定の気中微生物情報」を獲得する「エアロゾル・フローサイトメトリー」の実現を目指し，「気中微生物の蛍光染色」の可能性を検証すると伴に装置開発を試みた。エアロゾル機器で必須の「短時間の微生物染色の実現の可否」，気中微生物・蛍光染料ミスト混合による「エアロゾル染色実現の可否」，染色効率化のための「エレクトロスプレー染料粒子生成」を検討し，適切な条件設定で「10秒以内」の染色が可能なこと，「エアロゾル混合による染色」を検証し，試作装置による安定したエレクトロスプレー染料粒子生成を確認した。これは世界で初めての「気中微生物の浮遊状態での蛍光染色」の成功例となる。","subitem_description_type":"Abstract"},{"subitem_description":"A rapid method for the online detection of bio-aerosols could be useful for the efficient management of areas that are contaminated with bio-aerosols. Airborne microbes of interest can be stained by exposing them to a fluorescent dye in the form of a mist, and the resulting stained microbes can be detected by an optical detection method. To accomplish this, we have tested the possibility as follows:\n1) The rapid staining of yeast (S. cerevisiae) in an aqueous solution of typical dyes, such as DAPI and Auramine-O, was examined using a fluorescence spectrophotometer. The relaxation time, which corresponds to ~63.2 % of the maximum fluorescence intensity, could be adjusted to below 10 seconds.\n2) Mixtures of aerosols of microbes and a mist of a fluorescent dye solution were examined as a possible method for staining microbes. By simply mixing an aerosol yeast and dye mist, a certain fraction of yeast could be confirmed to be stained.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:16H02934, 研究期間(年度):2016-04-01 - 2019-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「気中微生物オンライン測定のためのエアロゾル・フローサイトメトリーの開発」研究成果報告書　課題番号16H02934\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16H02934/16H02934seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00053981","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学理工研究域地球社会基盤学系"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=70165463"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=70165463","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16H02934/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16H02934/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16H02934/16H02934seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16H02934/16H02934seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-04-13"}],"displaytype":"detail","filename":"TE-PR-FURUUCHI-M-kaken 2019-4p.pdf","filesize":[{"value":"155.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"TE-PR-FURUUCHI-M-kaken 2019-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/47657/files/TE-PR-FURUUCHI-M-kaken 2019-4p.pdf"},"version_id":"4d206b7e-0c06-4cd9-b357-93bae66e434e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"気中微生物オンライン測定のためのエアロゾル・フローサイトメトリーの開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"気中微生物オンライン測定のためのエアロゾル・フローサイトメトリーの開発"},{"subitem_title":"Development of an aerosol flow cytometry technology for online measurement of airborne microorganisms","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2815"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-04-13"},"publish_date":"2020-04-13","publish_status":"0","recid":"47657","relation_version_is_last":true,"title":["気中微生物オンライン測定のためのエアロゾル・フローサイトメトリーの開発"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T13:40:38.452580+00:00"}