{"created":"2023-07-27T06:53:30.240344+00:00","id":48255,"links":{},"metadata":{"_buckets":{"deposit":"5e97a8b4-bf94-40a1-a9f1-067315e4b797"},"_deposit":{"created_by":18,"id":"48255","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"48255"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00048255","sets":["2812:2813:2815"]},"author_link":["247","85968"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-05-27","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2016-04-01 - 2019-03-31","bibliographic_titles":[{"bibliographic_title":"平成30(2018)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2018 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"我々はCRISPR/Cas9システムを用いてSatb1の欠損細胞株を樹立した。Satb1欠損F12細胞にて，15q11-q13領域のクロマチン脱凝集状態を解析した所，正常細胞株と比較し脱凝集が小さくなっていることが明らかとなった。一方，Satb1を強制発現させたF12細胞では，クロマチン脱凝集状態が正常に比べ大きく広がっていることを見出した。このことから，15q11-q13領域のクロマチン脱凝集の構築には，Satb1が大きく関わっていることが明らかとなった。また， Satb1の欠損細胞株において，15q11-q13領域のMAGEL2/NDNの発現が低下していることが明らかとなった。 ","subitem_description_type":"Abstract"},{"subitem_description":"We established Satb1 deficient cell line using the CRISPR / Cas9 system. Analysis of chromatin disaggregation in the 15q11-q13 region of Satb1-deficient F12 cells revealed smaller disaggregation compared to normal cell lines. On the other hand, in F12 cells in which Satb1 was forcedly expressed, it was found that the chromatin disaggregation state spreads more than normal. From this, it became clear that Satb1 is greatly involved in the construction of chromatin disaggregation in the 15q11-q13 region. In addition, it was revealed that the expression of MAGEL2 / NDN in the 15q11-q13 region is reduced in the Satb1 deficient cell line. ","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:16K07196, 研究期間(年度):2016-04-01 - 2019-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「LINE1配列のストランド特異的分布とMARを介したクロマチン制御機構の解明」課題番号16K07196\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K07196/16K07196seika/)を加工して作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00054577","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学学際科学実験センター"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=40448311"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40448311","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K07196/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K07196/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K07196/16K07196seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K07196/16K07196seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-04-23"}],"displaytype":"detail","filename":"FR-PR-HORIKE-S-kaken 2019-4p.pdf","filesize":[{"value":"2.0 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"FR-PR-HORIKE-S-kaken 2019-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/48255/files/FR-PR-HORIKE-S-kaken 2019-4p.pdf"},"version_id":"747d396f-f6f8-433f-867c-14fdfeeb3e19"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"LINE1配列のストランド特異的分布とMARを介したクロマチン制御機構の解明","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"LINE1配列のストランド特異的分布とMARを介したクロマチン制御機構の解明"},{"subitem_title":"The role of strand specific distribution of LINE1 sequence in chromatin regulation","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2815"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-04-23"},"publish_date":"2020-04-23","publish_status":"0","recid":"48255","relation_version_is_last":true,"title":["LINE1配列のストランド特異的分布とMARを介したクロマチン制御機構の解明"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T13:36:14.533784+00:00"}