{"created":"2023-07-27T06:53:30.512987+00:00","id":48262,"links":{},"metadata":{"_buckets":{"deposit":"f5ed3b7e-70dc-45de-ada7-efbbdf10be6d"},"_deposit":{"created_by":18,"id":"48262","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"48262"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00048262","sets":["2812:2813:3929"]},"author_link":["585","21035"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-05-22","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"13p.","bibliographicVolumeNumber":"2016-04-01 - 2020-03-31","bibliographic_titles":[{"bibliographic_title":"令和1(2019)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2019 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"パルミチン酸 (PA)及びエイコサペンタエン酸 (EPA)がインスリン抵抗性誘導セレノプロテインP (SeP)発現に及ぼす影響を検討。H4IIEC肝細胞において、Sepp1遺伝子発現はPAにより亢進し、EPAにより減少。SEPP1プロモーター上の応答配列はPA及びEPAに対して共通で、Sterol regulatory element (SRE)-likeな配列を有する。PAはSREBP-1c経路非依存的にSeP発現を誘導、EPAはSREBP-1c経路依存的にSeP発現を抑制。EPAはSREBP-1cの不活化を介し肝臓でのSeP発現を抑制し、全身のインスリン抵抗性を改善する可能性がある。","subitem_description_type":"Abstract"},{"subitem_description":"Selenoprotein P (encoded by SELENOP in humans, Selenop in rat), a liver-derived secretory protein, induces resistance to insulin and VEGF in type 2 diabetes. The present findings demonstrate that EPA suppresses SELENOP expression by inactivating SREBP-1c in H4IIEC3 hepatocytes. Treatment with EPA caused concentration- and time-dependent reduction in SELENOP promoter activity. EPA activated AMPK; however, the inhibitory effect of EPA on SELENOP promoter activity was not canceled with an AMPK inhibitor compound C and dominant-negative AMPK transfection. Deletion mutant promoter assays and computational analysis of transcription factor-binding sites conserved among the species resulted in identification of a SRE-like site in the SELENOP promoter. A ChIP assay revealed that EPA decreases binding of SREBP-1c to the SELENOP promoter. Knockdown of Srebf1 resulted in a significant down-regulation of Selenop expression. SREBP-1c overexpression inhibited the suppressive effect of EPA.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:16K09739, 研究期間(年度):2016-04-01 - 2020-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「セレノプロテインPを介した脂肪酸によるインスリン抵抗性回復機序の解明」研究成果報告書　課題番号16K09739\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K09739/16K09739seika/)を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学附属病院内分泌・代謝内科","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00054584","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=40507042"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40507042","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K09739/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K09739/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K09739/16K09739seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K09739/16K09739seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-04-16"}],"displaytype":"detail","filename":"ME-PR-TAKESHITA-Y-kaken 2020-13p.pdf","filesize":[{"value":"203.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-TAKESHITA-Y-kaken 2020-13p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/48262/files/ME-PR-TAKESHITA-Y-kaken 2020-13p.pdf"},"version_id":"f3f4dcbd-0e3b-4c46-81c3-2a3987d3f7ad"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"セレノプロテインPを介した脂肪酸によるインスリン抵抗性回復機序の解明","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"セレノプロテインPを介した脂肪酸によるインスリン抵抗性回復機序の解明"},{"subitem_title":"Mechanism of insulin resistance by fatty acids via selenoprotein P","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["3929"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-04-16"},"publish_date":"2021-04-16","publish_status":"0","recid":"48262","relation_version_is_last":true,"title":["セレノプロテインPを介した脂肪酸によるインスリン抵抗性回復機序の解明"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:02:43.773878+00:00"}