{"created":"2023-07-27T06:53:43.006550+00:00","id":48555,"links":{},"metadata":{"_buckets":{"deposit":"154aff1b-6fa2-49bd-b24e-65b547a3b6cb"},"_deposit":{"created_by":18,"id":"48555","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"48555"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00048555","sets":["2812:2813:2815"]},"author_link":["86426","2328"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-05-16","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2017-06-30 - 2019-03-31","bibliographic_titles":[{"bibliographic_title":"平成30(2018)年度 科学研究費補助金 挑戦的研究(萌芽) 研究成果報告書"},{"bibliographic_title":"2018 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ゲノム編集技術を用いて，ヒトグリオーマで認められる主要遺伝子変異（CDKN2A，PTEN，NF1，TP53）を初代培養アストロサイトに導入したところ，不死化や増殖能の亢進が観察された。しかし，この不死化アストロサイトは，患者由来サンプルと比較して，僅かなスフィア形成能を有するのみであった。既知の幹細胞性獲得転写因子の強制発現は，分化した脳腫瘍細胞においては未分化性獲得に寄与したものの，アストロサイトでは不十分であり，更なる遺伝子異常あるいはシグナル異常が必要であると考えられた。一方，本研究において，新規の幹細胞可視化システムを構築したため，今後の未知因子の探索に有用であると期待された。 ","subitem_description_type":"Abstract"},{"subitem_description":"In this study, we aimed to understand relationship between genomic abnormality and transcription activity for acquisition of stem cell property of brain tumor. We found that primary human astrocytes were immortalized by introducing gene mutations of 4 major tumor suppressors or oncogenes, however, they did not form remarkable tumor mass in vivo, unlike patient-derived glioma samples. In addition, introduction of 4 transcription factors which reportedly contribute to acquisition of stem cell property of differentiated glioma cells were not able to transform immortalized astrocytes into malignant status, indicating that additional mutations are required. In the paralleled experiments, we successfully generated a patient-derived glioma cell clone, in which GFP was inserted at the locus of a stem cell marker gene. Since this GFP system is useful for visualization of stemness of tumor, we expect that it contributes to discovery of factors essential for acquisition of cancer stemness. ","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:17K19585, 研究期間(年度):2017-06-30 - 2019-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「がん未分化性獲得過程におけるゲノム異常と転写調節のクロストーク」課題番号17K19585\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17K19585/17K19585seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00054877","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学がん進展制御研究所"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=90343350"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=90343350","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K19585/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K19585/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17K19585/17K19585seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17K19585/17K19585seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-04-27"}],"displaytype":"detail","filename":"CA-PR-HIRAO-A-kaken 2019-4p.pdf","filesize":[{"value":"84.6 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-HIRAO-A-kaken 2019-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/48555/files/CA-PR-HIRAO-A-kaken 2019-4p.pdf"},"version_id":"a84080e2-66a3-4b26-8063-ea320f41b96f"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"がん未分化性獲得過程におけるゲノム異常と転写調節のクロストーク","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"がん未分化性獲得過程におけるゲノム異常と転写調節のクロストーク"},{"subitem_title":"Crosstalk between genomic abnormality and transcription activity for acquisition of stem cell property of cancer","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2815"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-04-27"},"publish_date":"2020-04-27","publish_status":"0","recid":"48555","relation_version_is_last":true,"title":["がん未分化性獲得過程におけるゲノム異常と転写調節のクロストーク"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T13:33:49.706320+00:00"}