{"created":"2023-07-27T06:53:45.836087+00:00","id":48619,"links":{},"metadata":{"_buckets":{"deposit":"4ff5724a-24ad-477a-a7e7-1f5bc0cfff28"},"_deposit":{"created_by":18,"id":"48619","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"48619"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00048619","sets":["2812:2813:2820"]},"author_link":["86506","86507"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-06-10","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5p.","bibliographicVolumeNumber":"2012-04-01 - 2014-03-31","bibliographic_titles":[{"bibliographic_title":"平成25(2013)年度 科学研究費補助金 若手研究(B) 研究成果報告書"},{"bibliographic_title":"2013 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"細胞膜電位コントロールデバイスを開発し，これを用いてプレスチンを発現させた培養細胞の電気特性計測及び単離細胞膜表面の微細構造観察を試みた．その結果，デバイス基板の微小孔（直径約2 um）上に，細胞をポジショニングすることに成功し，細胞膜表面の詳細構造の可視化にも成功した．さらに，デバイス基板の微小孔上に，人工脂質膜を作製することを試み，脂質膜形成後にギガオームシールが形成されることが確認された．このことは，当該デバイスで脂質膜中に再構成したタンパク質の変形挙動を可視化できる可能性を示唆している．今後，得られた知見を基に，プレスチンの変形挙動の可視化に取り組む．","subitem_description_type":"Abstract"},{"subitem_description":"A cell membrane potential control device was developed. Using this device, the microstructure and electrophysiological properties of prestin-expressing culture cells were investigated. A cell was successfully positioned on a micropore of the device (about 2 um in diameter) and the microstructure of the cell surface was visualized. In addition, an artificial lipid bilayer was created on the micropore, resulting in the formation of a gigaohm seal. These results suggest that it may be possible to visualize protein reconstituted in the lipid bilayer by using this device. Based on these findings, further research to visualize the conformational change of prestin will be performed.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:24791739, 研究期間(年度):2012-04-01 - 2014-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「新規パッチクランプ原子間力顕微鏡によるPrestin構造変化の分子メカニズム解明」研究成果報告書　課題番号24791739\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24791739/24791739seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00054941","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学理工研究域フロンティア工学系 / 鹿児島大学"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=70570901"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=70570901","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791739/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791739/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24791739/24791739seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24791739/24791739seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-18"}],"displaytype":"detail","filename":"FR-PR-MURAKOSHI-M-kaken 2014-5p.pdf","filesize":[{"value":"2.6 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"FR-PR-MURAKOSHI-M-kaken 2014-5p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/48619/files/FR-PR-MURAKOSHI-M-kaken 2014-5p.pdf"},"version_id":"f813d186-3e60-41e7-a2ee-3fcc51f7d2ed"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"新規パッチクランプ原子間力顕微鏡によるPrestin構造変化の分子メカニズム解明","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"新規パッチクランプ原子間力顕微鏡によるPrestin構造変化の分子メカニズム解明"},{"subitem_title":"Study of molecular mechanisms of structural change of prestin using a novel atomic force microscope combined with a patch clamp system","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2820"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-07-18"},"publish_date":"2019-07-18","publish_status":"0","recid":"48619","relation_version_is_last":true,"title":["新規パッチクランプ原子間力顕微鏡によるPrestin構造変化の分子メカニズム解明"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T09:10:58.437406+00:00"}