{"created":"2023-07-27T06:54:02.870640+00:00","id":49170,"links":{},"metadata":{"_buckets":{"deposit":"aad18fc3-74be-44aa-bef0-8c39cd883dfc"},"_deposit":{"created_by":18,"id":"49170","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"49170"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00049170","sets":["2812:2813:2830"]},"author_link":["88087","88086"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"8p.","bibliographicVolumeNumber":"2002-2003","bibliographic_titles":[{"bibliographic_title":"平成15(2003)年度 科学研究費補助金 基盤研究(B) 研究成果報告書"},{"bibliographic_title":"2003 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ADAMTS-1は、トロンボスポンジン(TSP)タイプIモチーフを持つADAM型メタロプロテアーゼである。我々はこれまでADAMTS-1が、軟骨の主要なプロテオグリカンであるアグリカンを分解する活性を有していることを明らかにしてきた。ADAMTS-1によるアグリカン切断部位は、関節炎患者の関節滑液中で検出されるin vivoでのアグリカン断片のものと一致することから、ADAMTS-1がアグリカンの分解を介することによって、関節炎における軟骨組織破壊に関わっている可能性が考えられる。本研究では、ADAMTS-1遺伝子欠損マウスでコラーゲン誘導性関節炎を誘発し、野生型マウスと比較して関節軟骨破壊の軽減が見られるかどうかを調べることにより、関節炎誘発時の関節軟骨破壊におけるADAMTS-1の役割を調べることを目的としている。この目的のためADAMTS-1遺伝子欠損マウスをコラーゲン誘導性関節炎に感受性を示すDBA/1マウスと戻し交配を10世代行い、現在実験に必要な繁殖を行っている段階である。この間、ADAMTS-1遺伝子欠損マウスを用いて、平行して進めているADAMTS-1の卵巣機能における役割の解析を先に行った。まず、3週令雌マウスにPMSGおよびhCGを投与する過排卵条件下で排卵数を測定した場合、ADAMTS-1遺伝子欠損雌マウスでは、ヘテロマウスと比較して、排卵数の著しい低下が認められた。このように、ADAMTS-1遺伝子欠損マウスでは、卵巣全体の機能が低下していることから、ADAMTS-1が卵巣機能において、必須な役割を果たしていることが示唆された。現在、排卵過程および卵胞成熟過程にどのように関わっているか検討を行っている。","subitem_description_type":"Abstract"},{"subitem_description":"ADAMTS-1 is an ADAM family protein with thrombospondin(ISP) type I motifs. By analyzing ADAMTS-1 gene knockout mice, we have shown that ADAMTS-1 is a multifunctional metalloproteinase that is essential for the structure and function of the ureteropelvic junction and adrenal glands as well as of the female genital organs. In addition, we previously demonstrated that ADAMTS-1 is able to cleave a major cartilage proteoglycan, aggrecan. Since the ADAMTS-1 cleavage sites of aggrecan correspond to the in vivo cleavage sites of aggrecan, which have been identified from aggrecan fragments in the synovial fluid of arthritis patients, it is possible that ADAMTS-1 may be involved in the degradation of cartilage tissue during arthritis through the degradation of aggrecan in vivo. The purpose of this study is to clarify the role of ADAMTS-1 on the degradation of cartilage in a collagen-induced arthritis model. ADAMTS-1(-/-) mice were backcrossed to DBA1 mice for ten generations and we are in the process of breeding knockout mice, needed for this experiment. In the meantime, examination of the ovarian function of ADAMTS-1(-/-) female mice has been preceding because it was found that fertilization was severely impaired in ADAMTS-1(-/-) females. When 3-week-old female mice were treated with PMSG and hCG, ADAMTS-1(-/-) females ovulated very few oocytes, approximately one-eighth the number ovulated by ADAMTS-1(+/-), indicating that the overroll ovarian function of ADAMTS-1(-/-) female mice is impaired. This finding suggests that ADAMTS-1 is essential for the ovarian function.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:14570182, 研究期間(年度):2002-2003","subitem_description_type":"Other"},{"subitem_description":"出典：「ノックアウトマウスを用いたADAMTS-1の骨代謝における役割の解析」研究成果報告書　課題番号14570182\n  (KAKEN：科学研究費助成事業データベース（国立情報学研究所）)\n　　　本文データは著者版報告書より作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00055483","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学がん進展制御研究所"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=40242565"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40242565","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14570182/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14570182/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-14570182/145701822003kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-14570182/145701822003kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-09-27"}],"displaytype":"detail","filename":"CA-PR-KUNO-K-kaken 2004-8p.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-KUNO-K-kaken 2004-8p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/49170/files/CA-PR-KUNO-K-kaken 2004-8p.pdf"},"version_id":"c63eb132-881e-4976-bcc3-9c0792b3d9ea"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ノックアウトマウスを用いたADAMTS-1の骨代謝における役割の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ノックアウトマウスを用いたADAMTS-1の骨代謝における役割の解析"},{"subitem_title":"Study of function of ADAMTS-1 in bone metabolism","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2830"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-09-27"},"publish_date":"2019-09-27","publish_status":"0","recid":"49170","relation_version_is_last":true,"title":["ノックアウトマウスを用いたADAMTS-1の骨代謝における役割の解析"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:37:01.916528+00:00"}