{"created":"2023-07-27T06:54:03.920779+00:00","id":49209,"links":{},"metadata":{"_buckets":{"deposit":"660da569-94b0-48ff-8cf9-b767c10c6471"},"_deposit":{"created_by":18,"id":"49209","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"49209"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00049209","sets":["2812:2813:2815"]},"author_link":["2451","88142"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-05-20","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5p.","bibliographicVolumeNumber":"2016-04-01 - 2019-03-31","bibliographic_titles":[{"bibliographic_title":"平成30(2018)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2018 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"増殖因子受容体に対する人工リガンド技術は疾患や再生医療に応用可能である。本研究では環状ペプチドスクリーニングを用いて、インスリン受容体に対する人工リガンドの取得と、人工アゴニスト環状ペプチドの性状に関する基盤研究を行った。組換えIRタンパク質は活性を保ったままでの生成が困難であり、ナノディスクによる可溶化IRの精製を試行している。環状ペプチドアゴニストが本来のリガンドとほとんど同じ活性を持ち得ること、不完全アゴニスト環状ペプチドを用いることで本来のリガンドと異なる選択的な細胞応答性を誘導できること、ユニークな環状ペプチドータンパク質の結合様式を立証し、人工リガンド技術確立のための知見を得た。","subitem_description_type":"Abstract"},{"subitem_description":"Artificial ligands for growth factor receptors are clinically applicable. We here tried identification of artificial ligands for insulin receptor (IR) using macrocyclic peptide screening, and characterized MET-agonist macrocyclic peptides to establish artificial ligand technology based on macrocyclic peptide. Expression and purification of IR protein with its kinase activity was difficult. Now, purification of IR in nanodiscs is being investigated. We have proved that 1) artificial ligands based on macrocyclic peptide are able to have similar activity compared to natural ligands, 2) partial agonists based on macrocyclic peptide are able to induced biased or selective cellular responses compared to natural ligands, and 3) macrocyclic peptides interact with thier target protein in an unique manner. These results illustrate potential of artificial ligands based on macrocyclic peptides. ","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:16K08544, 研究期間(年度):2016-04-01 - 2019-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「リガンド受容能不全の変異ペプチドホルモン受容体に対する人工リガンド創製の基盤研究」課題番号16K08544\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K08544/16K08544seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00055522","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学がん進展制御研究所"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=10523318"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=10523318","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K08544/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K08544/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K08544/16K08544seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K08544/16K08544seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-05-14"}],"displaytype":"detail","filename":"CA-PR-SAKAI-K-kaken 2019-5p.pdf","filesize":[{"value":"169.9 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-SAKAI-K-kaken 2019-5p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/49209/files/CA-PR-SAKAI-K-kaken 2019-5p.pdf"},"version_id":"adf56481-5134-4ad1-a1b8-baf0f96946d2"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"リガンド受容能不全の変異ペプチドホルモン受容体に対する人工リガンド創製の基盤研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"リガンド受容能不全の変異ペプチドホルモン受容体に対する人工リガンド創製の基盤研究"},{"subitem_title":"Establishment of artificial ligands technology for peptide hormone receptors with functional mutations","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2815"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-05-14"},"publish_date":"2020-05-14","publish_status":"0","recid":"49209","relation_version_is_last":true,"title":["リガンド受容能不全の変異ペプチドホルモン受容体に対する人工リガンド創製の基盤研究"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T13:32:36.670081+00:00"}