{"created":"2023-07-27T06:54:14.974764+00:00","id":49439,"links":{},"metadata":{"_buckets":{"deposit":"1e0853ca-f236-4d19-866e-6b23d2d33734"},"_deposit":{"created_by":18,"id":"49439","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"49439"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00049439","sets":["2812:2813:2816"]},"author_link":["88894","88895"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-06-11","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2015-04-01 - 2018-03-31","bibliographic_titles":[{"bibliographic_title":"平成29(2017)年度 科学研究費補助金 若手研究(B) 研究成果報告書"},{"bibliographic_title":"2017 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ホモ接合体性家族性高コレステロール血症(HoFH)患者(c.901G>T) の末梢静脈血から疾患特異的iPS細胞の樹立を行い、肝細胞への分化誘導および肝細胞に特異的なマーカーの遺伝子発現の確認を行なった。その後、LDL受容体遺伝子などの脂質代謝に関連する遺伝子の発現や機能解析を行った。CRISPR/Cas9システムを用いたゲノム編集を行い、HoFH患者を由来とするiPS細胞より、ホモ接合性およびヘテロ接合性に野生型と同じ配列を持つiPSクローンを樹立した。肝細胞への分化誘導後、LDL取り込み能に関する解析およびHoFH患者末梢血単核球への免疫反応について検討を行った。","subitem_description_type":"Abstract"},{"subitem_description":"To examine whether LDLR function of HLCs from genetically-corrected iPSCs can be restored in human, iPSCs were generated from a HoFH patient harboring a point mutation (c.901G>T) in exon 6 of LDLR, which impairs LDL uptake. We generated one homozygous gene-corrected HoFH-iPSC clone and two heterozygous gene-corrected HoFH-iPSC clones using the CRISPR/Cas9 system. Subsequently, we obtained functionally corrected HLCs with significantly recovered LDL uptake ability compared to those without gene correction. Under these conditions, few immunological rejections were observed by cell-mediated cytotoxicity assay.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:15K19508, 研究期間(年度):2015-04-01 - 2018-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「ヒトiPS細胞由来肝細胞を用いた家族性高コレステロール血症に対する移植治療の検討」課題番号15K19508\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K19508/15K19508seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学附属病院救急部","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00055751","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=10735161"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=10735161","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K19508/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K19508/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K19508/15K19508seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K19508/15K19508seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-11"}],"displaytype":"detail","filename":"ME-PR-OKADA-H-kaken 2018-4p.pdf","filesize":[{"value":"97.8 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-OKADA-H-kaken 2018-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/49439/files/ME-PR-OKADA-H-kaken 2018-4p.pdf"},"version_id":"94f81776-2942-49c5-99f8-8b7d6cca7e16"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ヒトiPS細胞由来肝細胞を用いた家族性高コレステロール血症に対する移植治療の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ヒトiPS細胞由来肝細胞を用いた家族性高コレステロール血症に対する移植治療の検討"},{"subitem_title":"Restored Low Density Lipoprotein Uptake in Gene-correctde iPSC-derived Hepatocytes from Homozygous Familial Hypercholesterolemia","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2816"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-10-11"},"publish_date":"2019-10-11","publish_status":"0","recid":"49439","relation_version_is_last":true,"title":["ヒトiPS細胞由来肝細胞を用いた家族性高コレステロール血症に対する移植治療の検討"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:49:30.565061+00:00"}