{"created":"2023-07-27T06:54:15.021636+00:00","id":49440,"links":{},"metadata":{"_buckets":{"deposit":"2976569d-12ec-40e1-a3b7-b6f8e34f5346"},"_deposit":{"created_by":18,"id":"49440","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"49440"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00049440","sets":["2812:2813:2815"]},"author_link":["88896","69787"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-06-11","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2017-04-01 - 2019-03-31","bibliographic_titles":[{"bibliographic_title":"平成30(2018)年度 科学研究費補助金 若手研究(B) 研究成果報告書"},{"bibliographic_title":"2018 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"エメリードレフュス型筋ジストロフィー（EDMD）の病態解明のため、原因蛋白であるエメリン、ラミン、ネスプリンをノックダウンし、核膜陥入層の評価、及び本疾患のメカニズムの解明を行った。\nエメリン、ラミンA/Cをノックダウンさせたラット胎仔心筋、EDMD由来のiPS細胞を作製した。エメリン、ラミンA/Cの発現量が減少することで、核膜陥入層の減少、及び核面積が増大が認められたことより、核の構造維持にラミンA/Cが関与している可能性が示唆された。また、pERKの発現量については有意差はみられなかったものの、血清存在下にてHDAC3の核内の分布が低下することが示された。","subitem_description_type":"Abstract"},{"subitem_description":"Emery-Dreifuss muscular dystrophy (EDMD) is caused by nuclear envelope protein such as emerin, lamin and nesprin. To examine the role of lamin, emerin and nesprin in cardiomyocyte, we produced EDMD-iPS derived cardiomyocyte, rat neonate cardiomyocyte during siRNA-mediated lamin, emerin and nesprin.\nThe incidence of cardiomyocyte with nuclear invagination was significantly decreased and the nuclear size was significantly increased. These data suggest that lamin has a pivotal function in the maintenance of nuclear invagination and nuclear size. To clarify mechanism of EDMD, the expression of pERK and HDAC3 was assesed. Although the expression of pERK was not changed significantly, the distribution of HDAC3 in nuclear was decreased under horse serum condition. ","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:17K16244, 研究期間(年度):2017-04-01 - 2019-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「Emery-Dreifuss型筋ジストロフィーの核膜構造と核内Ca動態の検討」課題番号17K16244\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17K16244/17K16244seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00055752","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学附属病院救急部"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=00613754"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=00613754","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K16244/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K16244/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17K16244/17K16244seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17K16244/17K16244seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-05-14"}],"displaytype":"detail","filename":"HO-PR-SHIMOJIMA-M-kaken 2019-4p.pdf","filesize":[{"value":"283.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"HO-PR-SHIMOJIMA-M-kaken 2019-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/49440/files/HO-PR-SHIMOJIMA-M-kaken 2019-4p.pdf"},"version_id":"a0ca77ea-df4b-442e-8c87-d3d42e9b0aa9"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"Emery-Dreifuss型筋ジストロフィーの核膜構造と核内Ca動態の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Emery-Dreifuss型筋ジストロフィーの核膜構造と核内Ca動態の検討"},{"subitem_title":"Structure of nuclear envelope and Nuclear calcium transient of Emery Dreifuss muscular dystrophy","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2815"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-05-14"},"publish_date":"2020-05-14","publish_status":"0","recid":"49440","relation_version_is_last":true,"title":["Emery-Dreifuss型筋ジストロフィーの核膜構造と核内Ca動態の検討"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T13:32:29.550021+00:00"}