{"created":"2023-07-27T06:54:30.406703+00:00","id":49853,"links":{},"metadata":{"_buckets":{"deposit":"33c8e581-8689-413b-ac98-8d9add495859"},"_deposit":{"created_by":18,"id":"49853","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"49853"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00049853","sets":["2812:2813:2818"]},"author_link":["89754"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-06-09","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5p.","bibliographicVolumeNumber":"2014-04-01 - 2016-03-31","bibliographic_titles":[{"bibliographic_title":"平成27(2015)年度 科学研究費補助金 若手研究(B) 研究成果報告書"},{"bibliographic_title":"2015 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"発酵阻害物質であるフルフラールのアミノ酸生産菌の増殖と発酵に対する影響を調べた。その結果、増殖は影響を受けるが、時間経過とともにフルフリルアルコールと2-フランカルボン酸に変換されることが分かった。遺伝子欠損株の解析から、フルフラールをフルフリルアルコールに変換する遺伝子を見出した。本遺伝子の精製タンパク質は試験管内でNADPHを補因子にフルフラールをフルフリルアルコールに変換した。\nまた、酸素を代謝スイッチとして好気条件下でアミノ酸のリジンを、嫌気条件下で有機酸であるコハク酸を同一株で生産する代謝改変株を作製した。","subitem_description_type":"Abstract"},{"subitem_description":"We firstly investigated cell growth of amino acid producing Corynebacterium glutamicum under furfural stress. The growth was inhibited by furfural, but furfural was degraded into furfuryl alcohol and 2-furoic acid overtime. Through constructing deletion strains of alcohol dehydrogenase genes, we identified the gene responsible for the conversion of furfural into furfuryl alcohol. Purified protein of this gene converted furfural into furfuryl alcohol in vitro using NADPH as a co-factor.\nWe moreover constructed a strain by metabolic engineering producing amino acid lysine under aerobic conditions and organic acid succinic acid under anaerobic conditions using oxygen as a metabolic switch.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:26870363, 研究期間(年度):2009-2010","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「汎用的な細菌の凝集誘導システムの開発とそれを利用した二段階物質生産システムの構築」課題番号:26870363\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26870363/26870363seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学新学術創成研究機構 / 神戸大学","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00056165","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=00647422"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=00647422","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26870363/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26870363/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26870363/26870363seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26870363/26870363seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-11-22"}],"displaytype":"detail","filename":"FR-PR-TSUGE-Y-kaken 2016-5p.pdf","filesize":[{"value":"603.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"FR-PR-TSUGE-Y-kaken 2016-5p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/49853/files/FR-PR-TSUGE-Y-kaken 2016-5p.pdf"},"version_id":"580418a7-7935-4e87-bfc1-f13347aab2a6"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"汎用的な細菌の凝集誘導システムの開発とそれを利用した二段階物質生産システムの構築","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"汎用的な細菌の凝集誘導システムの開発とそれを利用した二段階物質生産システムの構築"},{"subitem_title":"Construction of versatile flocculation system and its application for two-stage production system","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2818"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-11-22"},"publish_date":"2019-11-22","publish_status":"0","recid":"49853","relation_version_is_last":true,"title":["汎用的な細菌の凝集誘導システムの開発とそれを利用した二段階物質生産システムの構築"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T10:49:32.946271+00:00"}