{"created":"2023-07-27T06:54:47.820456+00:00","id":50300,"links":{},"metadata":{"_buckets":{"deposit":"49309965-4042-4ace-8e1c-bcda17656bc0"},"_deposit":{"created_by":18,"id":"50300","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"50300"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00050300","sets":["2812:2813:2816"]},"author_link":["91101","91100"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-02-13","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2015-04-01 - 2018-03-31","bibliographic_titles":[{"bibliographic_title":"平成29(2017)年度 科学研究費補助金 挑戦的萌芽研究 研究成果報告書"},{"bibliographic_title":"2017 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takeuchi, Yutaka"}],"nameIdentifiers":[{"nameIdentifier":"91101","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"70418680","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70418680"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"RNA干渉法による不妊化アワビの種苗生産するための基盤技術の開発を行った。RNA干渉法に着目し、アワビの初期胚内で生殖細胞の発生に必須な母性因子を一時的に減少させた状態で発生させる条件を決定した。標的遺伝子として、vasa遺伝子を選定し、約300bpのdouble strand RNA (ds-RNA）を合成し、産卵直前のアワビ卵巣へ注入した。ds-RNAには、あらかじめDIG標識を施し、注入後のds-RNAの挙動を調べたところ、注入後3時間以降で、卵母細胞内に移行することが明らかとなった。さらに、このアワビを産卵させることで、受精卵内で母性vasa mRNA量を減少させることに成功した。","subitem_description_type":"Abstract"},{"subitem_description":"The sterilization of artificial seeds of abalone is an ideal strategy to promote faster growth and to avoid sexual precocity. Recently, RNA interference (RNAi) is the popular method for the production of sterile animals in vertebrates and invertebrates, and identification of target gene for this technology is a crucial step to the production of sterilization. To evaluate effects of vasa gene knockdown, the dispersion and inhibitor effect of vasa dsRNA in ovary were examined. After 12 hours post-injection, dsRNA with vasa DIG-labelled delivered to full-grown oocytes of female. Expression levels of vasa in RNAi group using 3 different dose of dsRNA were significantly lower than control group at 7 days post-injection (dpi) and 14 dpi. Similar inhibitor effects were observed in unfertilized eggs and 8-cell stage of embryos. The results suggest that RNAi of vasa gene can be effectively utilized in oocytes and early embryos of pacific abalone to reduce amount of mRNA level.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:15K14802, 研究期間(年度):2015-04-01 - 2018-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「RNA干渉法を用いた母性mRNAノックダウンによる不稔化エゾアワビ種苗の作出」課題番号15K14802\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K14802/15K14802seika/)を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学理工研究域生命理工学系 / 鹿児島大学 / 東京海洋大学","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00056609","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=70418680"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=70418680","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K14802/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K14802/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K14802/15K14802seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K14802/15K14802seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"竹内, 裕"}],"nameIdentifiers":[{"nameIdentifier":"91100","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"70418680","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70418680"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-27"}],"displaytype":"detail","filename":"SC-PR-TAKEUCHI-Y-kaken 2019-4p.pdf","filesize":[{"value":"687.6 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC-PR-TAKEUCHI-Y-kaken 2019-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/50300/files/SC-PR-TAKEUCHI-Y-kaken 2019-4p.pdf"},"version_id":"f0105136-3dcd-40da-b91a-1a39edfa85c0"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"RNA干渉法を用いた母性mRNAノックダウンによる不稔化エゾアワビ種苗の作出","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"RNA干渉法を用いた母性mRNAノックダウンによる不稔化エゾアワビ種苗の作出"},{"subitem_title":"Development of maternal mRNA knock-down system using double-strand RNA in abalone embryos","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2816"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-01-27"},"publish_date":"2020-01-27","publish_status":"0","recid":"50300","relation_version_is_last":true,"title":["RNA干渉法を用いた母性mRNAノックダウンによる不稔化エゾアワビ種苗の作出"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T05:35:56.632371+00:00"}