{"created":"2023-07-27T06:54:49.569663+00:00","id":50356,"links":{},"metadata":{"_buckets":{"deposit":"7ee530ad-c12c-4154-89d3-15713cf7b09e"},"_deposit":{"created_by":18,"id":"50356","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"50356"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00050356","sets":["2812:2813:2818"]},"author_link":["89019"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-06-14","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2014-04-01 - 2016-03-31","bibliographic_titles":[{"bibliographic_title":"平成27(2015)年度 科学研究費補助金 挑戦的萌芽研究 研究成果報告書"},{"bibliographic_title":"2015 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"マルチ銅オキシダーゼは基質から引き抜いた電子を三核銅部位に分子内長距離輸送し、共基質である酸素を４電子還元し水を生成する。本研究ではこの反応を逆向きに回転させることを目的とし、変異導入によってタイプ１銅の酸化還元電位を正電位シフトさせる戦略を立てた。CueOのタイプ１銅配位子及びその外圏に種々の改変を施し、タイプ１銅の酸化還元電位を正電位シフトさせることに成功し、様々の電極材料を用いて直接電気化学測定を行った。また、酸化還元電位が元来高いビリルビンオキシダーゼについて電気化学測定を行ったが、酸素の発生を示唆する電気化学応答は得られなかった。別の酵素について同様の検討を継続中である。","subitem_description_type":"Abstract"},{"subitem_description":"Multicopepr oxidase is a class of enzymes to perform the four-electron reduction of dioxygen at the trinuclear copper center by utilizing electrons from substrates extracted at the type I copper center. The aim of this study is to realize the reverse reaction of this four-electron reduction of dioxygen. Our strategy to realize this attempt was to shift the oxidation-reduction potential of the type I copper center towards positive direction by performing mutations at the ligands to type I copper and/or amino acids located in the outer coordination spheres. Mutations on CueO and bilirubin oxidase were succeeded but we could not realize the reverse reaction to form dioxygen even utilizing a variety of electrode materials. We are trying to realize our aim by using other multicopper oxidases.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:26650031, 研究期間(年度):2014-04-01 -2016-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「酵素過程の逆反応実現により水から還元力を取り出す試み」課題番号:26650031\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26650031/26650031seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学国際基幹教育院GS教育系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00056665","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=90116038"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=90116038","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26650031/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26650031/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26650031/26650031seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26650031/26650031seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-27"}],"displaytype":"detail","filename":"SC-PR-SAKURAI-T-kaken 2016-4p.pdf","filesize":[{"value":"283.7 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC-PR-SAKURAI-T-kaken 2016-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/50356/files/SC-PR-SAKURAI-T-kaken 2016-4p.pdf"},"version_id":"16814c05-cb59-4dcf-9f07-fd4d8cf6bb90"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"酵素過程の逆反応実現により水から還元力を取り出す試み","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"酵素過程の逆反応実現により水から還元力を取り出す試み"},{"subitem_title":"Attempts to obtain electrons from water molecules by realizing the reversed reaction of enzymatic process","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2818"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-01-27"},"publish_date":"2020-01-27","publish_status":"0","recid":"50356","relation_version_is_last":true,"title":["酵素過程の逆反応実現により水から還元力を取り出す試み"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T10:50:09.879385+00:00"}