{"created":"2023-07-27T06:54:49.618611+00:00","id":50357,"links":{},"metadata":{"_buckets":{"deposit":"c2653651-446b-465e-ace2-49427196e246"},"_deposit":{"created_by":18,"id":"50357","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"50357"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00050357","sets":["2812:2813:2817"]},"author_link":["89019"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-06-16","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"5p.","bibliographicVolumeNumber":"2014-04-01 - 2017-03-31","bibliographic_titles":[{"bibliographic_title":"平成28(2016)年度 科学研究費補助金 基盤研究(B) 研究成果報告書"},{"bibliographic_title":"2016 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"マルチ銅オキシダーゼによる酸素の４電子還元が共通した反応機構で進行することをCueO及びビリルビンオキシダーゼのタイプI銅配位子並びにプロトン輸送に関与するアミノ酸への変異導入によって明らかにした。この時、三核銅部位の外圏に配置されたアミノ酸が電子供与体として機能しないことがわかった。酸素還元過程の詳細をさらに詳しく解明するため中性子回折を行うことを計画し、重水中でCeuOを結晶化した。また、緩衝液起源の酢酸イオンがタイプII銅に結合することを見出した。さらに、タイプI銅の外圏部の改変により酵素活性を高め、なおかつ、優れた電極応答を実現し、生物燃料電池の電極触媒としての利用の道を開いた。","subitem_description_type":"Abstract"},{"subitem_description":"Studies on the three-domain multicopper oxidases such as CueO and bilirubin oxidase by the mutations at the type I copper ligand and/or the acidic amino acid located in the proton transport pathway revealed that the four-electron reduction of dioxygen proceeds according to a common reaction pathway. Amino acids located in the outer-sphere of the trinuclear copper center were found not to play a role as the fourth electron donor. From the finding that an acetate ion from buffer solution was bound to type II copper, it was found that this exogenous anion reaches deep inside protein molecule and enhances enzymatic activities. Further, we performed crystallization of CueO in heavy water for neutral diffraction to reveal the reaction mechanism. We also succeeded in enhancing enzymatic activities by performing mutations at the amino acids located in the outer-coordination spheres of type I copper and obtained excellent electrochemical responses suitable as cathodic enzyme for biofuel cell.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:26288076, 研究期間(年度):2014-04-01 - 2017-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：「マルチ銅オキシダーゼによる酸素還元機構の解明と機能改変体の電極触媒としての利用」研究成果報告書　課題番号26288076\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26288076/26288076seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学国際基幹教育院GS教育系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00056666","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=90116038"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=90116038","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26288076/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26288076/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26288076/26288076seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26288076/26288076seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-27"}],"displaytype":"detail","filename":"SC-PR-SAKURAI-T-kaken 2017-5p.pdf","filesize":[{"value":"896.4 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC-PR-SAKURAI-T-kaken 2017-5p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/50357/files/SC-PR-SAKURAI-T-kaken 2017-5p.pdf"},"version_id":"2d8f3397-27ac-46cf-9957-dc051bf77a06"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"マルチ銅オキシダーゼによる酸素還元機構の解明と機能改変体の電極触媒としての利用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"マルチ銅オキシダーゼによる酸素還元機構の解明と機能改変体の電極触媒としての利用"},{"subitem_title":"Study on Dioxygen Reduction Mechanism by Multicopper Oxidases and Application of Function Modulated Mutants as Electorode Catalyst","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2817"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-01-27"},"publish_date":"2020-01-27","publish_status":"0","recid":"50357","relation_version_is_last":true,"title":["マルチ銅オキシダーゼによる酸素還元機構の解明と機能改変体の電極触媒としての利用"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T11:14:10.923391+00:00"}