{"created":"2023-07-27T06:54:50.892585+00:00","id":50393,"links":{},"metadata":{"_buckets":{"deposit":"c2599653-1a11-4e95-b81e-bc8ddc3ecc1a"},"_deposit":{"created_by":18,"id":"50393","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"50393"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00050393","sets":["2812:2813:2816"]},"author_link":["91219","91218"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-06-01","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"2016-04-01 - 2018-03-31","bibliographic_titles":[{"bibliographic_title":"平成29(2017)年度 科学研究費補助金 挑戦的萌芽研究 研究成果報告書"},{"bibliographic_title":"2017 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"新しいゲノム編集技術であるCRISPR/Cas9法を用いて、Traj18欠損マウス（CRISPR-Traj18-/-）の樹立に成功した。CRISPR-Traj18-/-のT細胞レパトアは正常で、抗原提示分子CD1d拘束性のインバリアント・ナチュラルキラT（iNKT）細胞特異的欠損マウスの樹立に成功した。iNKT細胞の肥満における影響を明らかにするため、高脂肪食負荷試験を行った。その結果、CRISPR-Traj18-/-マウスでは野生型マウスと比べて体重の増加、インスリン抵抗性、グルコース応答性の減弱が認められ、iNKT細胞が肥満やそれに伴うⅠ型糖尿病の増悪に寄与していることが明らかとなった。","subitem_description_type":"Abstract"},{"subitem_description":"Here we have successfully generated new Traj18－/－ mouse lines by using the Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas9 technology. The CRISPR-Traj18－/－ mice lacked iNKT cells and harbored an undisturbed TCRα repertoire, fulfilling the criteria of iNKT cell-deficient mice.\nDivergent findings for the metabolic role of iNKT cells have been reported in studies using the previously generated Traj18－/－ mouse strain. Therefore, we re-investigated the contribution of iNKT cells to the development of obesity induced by a high-fat diet (HFD) using our novel Traj18－/－ mouse strain on the B6 background.Among the experimental groups on HFD, CRISPR-Traj18－/－ (1-1L) mice gained less weight than WT B6 mice. The CRISPR-Traj18－/－ mouse strain on HFD also exhibited ameliorated metabolic phenotypes, which is consistent with a pathogenic role of iNKT cells in the development of obesity and insulin-resistance.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:16K14591, 研究期間(年度):2016-04-01 - 2018-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「CRISPR/hCas9法による抗原特異的T細胞欠損マウスの作製と評価」課題番号16K14591\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K14591/16K14591seika/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系 / 東京大学","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00056702","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=70415339"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=70415339","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K14591/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K14591/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K14591/16K14591seika/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K14591/16K14591seika/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-27"}],"displaytype":"detail","filename":"ME-PR-WATARI-H-kaken 2018-4p.pdf","filesize":[{"value":"167.6 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-WATARI-H-kaken 2018-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/50393/files/ME-PR-WATARI-H-kaken 2018-4p.pdf"},"version_id":"c423818a-b3bb-463a-9553-44fe1b3d38d1"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"CRISPR/hCas9法による抗原特異的T細胞欠損マウスの作製と評価","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"CRISPR/hCas9法による抗原特異的T細胞欠損マウスの作製と評価"},{"subitem_title":"Generation and Analysis of Antigen-specific T cell-deficient mice by CRISPR/Cas9","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2816"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-01-27"},"publish_date":"2020-01-27","publish_status":"0","recid":"50393","relation_version_is_last":true,"title":["CRISPR/hCas9法による抗原特異的T細胞欠損マウスの作製と評価"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:50:53.352156+00:00"}