{"created":"2023-07-27T06:54:56.474253+00:00","id":50529,"links":{},"metadata":{"_buckets":{"deposit":"61ee18c3-7505-441d-927b-3e40e05eeecf"},"_deposit":{"created_by":18,"id":"50529","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"50529"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00050529","sets":["2812:2813:2833"]},"author_link":["44095","91487"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"29p.","bibliographicVolumeNumber":"1999-2000","bibliographic_titles":[{"bibliographic_title":"平成12(2000)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2000 Fiscal Year Final Research Report","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ラットにて一側黒質に6-ヒドロキシドーパミンを定位的に微量注入してドーパミン神経を選択的に破壊することでパーキンソンモデルを作成した。2週間後、アポモルフィンの腹腔内投与による回転運動を計測した後、断頭屠殺し脳の新鮮凍結切片を作成した。抗チロシンハイドロキシラーゼ(TH)抗体による免疫抗体染色を行い、定量した。[3H]SCH-23390を用いたドーパミンD1受容体、[3H]YM-09151-2によるドーパミンD2受容体および[3H]GBR-12935を用いたドーパミントランスポータ(DAT)の各オートラジオグラフィを専用イメージングプレートを使って行い、イメージングアナライザ(FUJI BAS-5000)で定量的に解析した。対照群では、生理食塩水を注入した。定量値は、それぞれ健側に対する破壊側の比で検討した。\nモデルラットの破壊側にてDAT結合と抗TH抗体による免疫抗体染色性が低下した。D2受容体の軽度上昇が認められたが、D1受容体の変化は無かった。これらは従来の報告に一致する。アポモルフィン投与による回転数と抗TH抗体染色性の低下との間には負の強い相関関係が認められた。DAT結合は、抗TH抗体染色性との間に弱い相関があったが、回転運動の評価や抗TH抗体染色性より鋭敏であった。破壊側のドーパミンD2受容体増加は回転運動が起きるような強い破壊でみられ、ドーパミン減少に伴う代償反応と考えられたが、THの低下しない弱い程度の破壊では増加が無くドーパミンの低下が乏しいことが推定される。DATによる画像化は、抗TH抗体の変化やアポモルフィンによる回転運動が起こるよりも軽度のドーパミン神経系の傷害を鋭敏に捉えることができる有用な方法であることが示された。ドーパミン神経の機能障害時においてドーパミン減少前に異常を捉えられて早期診断に役立つと思われる。","subitem_description_type":"Abstract"},{"subitem_description":"The aim of this study is to clarify the relationship between dopamine transporter autoradiography and tyrosine hydroxylase immunostaining in experimental unilateral parkinsonian model rat. Method : Parkinsonian model rats were stereotactically infused 6-OHDA into unilateral substantia nigra, and evaluated clockwise rotation behavior induced by administration of apomorphine. Sequential brain sections underwent immunostaining using anti-tyrosine hydroxylase (TH) antibody and autoradiography of dopaminergic system. Dopamine D1, D2 receptors and transporter density was assessed by imaging plate autoradiography using SCH, YM and GBR, respectively. Immunostained section were analyzed by optical digital imaging analyzer, and autoradiography were analyzed by imaging plate analyzer. Ratios of lesioned side value to contralateral side value in the caudate-putamen were used as indices. Correlations among apomorphine-induced rotation, TH immunostaining, dopamine D1, D2 receptors and transporter were analyzed statistically. Results : Significant positive correlations were shown between rotation, TH and GBR.GBR was more sensitive than TH and rotation. Conclusion : Dopamine transporter imaging is more sensitive than TH and rotation. This results support that dopamine transporter imaging may detect an early disease without obvious symptoms.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:11670877, 研究期間(年度):1999-2000","subitem_description_type":"Other"},{"subitem_description":"出典：「脳内ドーパミン作動性神経受容体及び節前線維機能の核医学的同時評価の基礎的研究」研究成果報告書　課題番号11670877\n  (KAKEN：科学研究費助成事業データベース（国立情報学研究所）)\n　　　本文データは著者版報告書より作成","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00056838","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_17":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"金沢大学医薬保健研究域保健学系"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=70227388"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=70227388","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670877/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670877/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-11670877/116708772000kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-11670877/116708772000kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-02-07"}],"displaytype":"detail","filename":"ME-PR-TUJI-S-kaken 2001-29p.pdf","filesize":[{"value":"10.3 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-TUJI-S-kaken 2001-29p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/50529/files/ME-PR-TUJI-S-kaken 2001-29p.pdf"},"version_id":"1d307cbe-307f-4b91-aba4-46a2ab4aee42"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"脳内ドーパミン作動性神経受容体及び節前線維機能の核医学的同時評価の基礎的研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"脳内ドーパミン作動性神経受容体及び節前線維機能の核医学的同時評価の基礎的研究"},{"subitem_title":"Basic research of simultaneous evaluation of dopaminergic receptor and presynaptic neuronal functions in the brain using autoradiography","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2833"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-02-07"},"publish_date":"2020-02-07","publish_status":"0","recid":"50529","relation_version_is_last":true,"title":["脳内ドーパミン作動性神経受容体及び節前線維機能の核医学的同時評価の基礎的研究"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T14:46:37.561046+00:00"}