{"created":"2023-07-27T06:54:58.012691+00:00","id":50568,"links":{},"metadata":{"_buckets":{"deposit":"e41e28e1-d628-4ca3-a383-93482582d575"},"_deposit":{"created_by":18,"id":"50568","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"50568"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00050568","sets":["2812:2813:2833"]},"author_link":["91488","20737"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-03-25","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1998-2000","bibliographic_titles":[{"bibliographic_title":"平成12(2000)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"},{"bibliographic_title":"2000 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"1 ラットの皮下に埋め込んだ滲透圧ポンプから側脳室内に合成βアミロイド蛋白(1-40)を300pg/dayで持続的に2週間注入して、アルツハイマー病モデルラットを作成した。モデルラットと対照ラットについて行動薬理学的変化(記憶学習障害の程度)を8方向放射状迷路を用いて評価した。さらに,ムスカリン性アセチルコリン(mACh)神経系の変化を,mACh受容体は[^3H]QNB,Achトランスポータは[^3H]vesamicolを用いて定量的オートラジオグラフィ(バイオイメージングアナライザーBAS5000にて定量解析)にて評価した。次にモデルラット作成(側脳室内の合成βアミロイド蛋白持続注入)と同時に,腹腔内に副腎皮質ホルモン(リン酸ベタメタゾンナトリウム)を1.2mg/2wを持続注入し,行動薬理学的及びベサミコールイメージングの改善の有無について評価した。\nモデル群では対照群と比較して迷路の正選択数が有意に低下したが,副腎皮質ホルモン投与により正選択数が増加した。[^3H]QNBの頭頂葉大脳皮質/小脳比はモデル群,対照群,副腎皮質投与群のいずれも有意差を認めなかった。一方,[^3H]vesamicolのトランスポータ結合量は対照群に比較してβアミロイド投与群で有意に低下し,副腎皮質ホルモン投与にて改善を示した。\n2 ラットの一側前脳基底核を破壊した学習障害モデルを作成し,迷走神経下神経節を破壊大脳皮質に自家移植して移植の効果について検討した。破壊群,移植群,シャム群に分けて,^<99m>Tc-HMPAOによる対局所脳血流,[^3H]QNB及び[^3H]vesamicolイメージングにて評価した。その結果,血流分布とmACh受容体の分布は有意差が認められなかったが,[^3H]ベサミコールは破壊群の患側皮質で有意に低下し,移植群では破壊群に比し,4週間後で有意な改善を認め,移植による効果が示された。","subitem_description_type":"Abstract"},{"subitem_description":"(1) In this study, β-amyloid protein was infused into the cerebral ventricle of rats for 14 days ; the eight-arm radial maze was used to evaluate memorial ability. In the same animals, vesicular acetylcholine transporter and muscarinic acetylcholine receptor density was measured. The performance of the eight-arm radial maze task was impaired in β-amyloid protein treated rats. In neocortex, the vesicular acetylcholine transporter density was lower in β-amyloid protein-treated rats than vehicle treated rats ; there was no difference in muscarinic acetylcholine receptor density between the two groups. These results suggest that the reduction in vesicular acetylcholine transporter density is related to memory impairment induced by β-amyloid protein.\n(2) It is considered that the nodosal ganglion grafting improves the learning and memory disorder in the nucleus basalis magnocellularis lesioned rat, and that [^3H]-vesamicol is one of the mapping agents for presynaptic cholinergic neurons. Quantitative autoradiography was performed using high sensitive imaging plate system to evaluate the effect of neurotransplantation on [^3H]-vesamicol binding. The affected-side to unaffected-side ratio of [^3H]-vesamicol in the nucleus basalis magnocellularis lesioned group was significantly lower than that in the sham-operated group at I , 2 and 4 weeks (p< 0.01). In the transplanted group, the ratio was significantly higher than that in the untreated group at 4 weeks (p<0.05). [^3H]-vesamicol binding is one of the most sensitive indicators of nucleus basalis magnocellularis lesions and the effect of the nodosal ganglion grafting.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:10670832, 研究期間(年度):1998-2000","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「アルツハイマー病における神経移植・薬物効果の核医学的評価に関する基礎的研究」課題番号1067083\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-10670832/106708322000kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学学際科学実験センター","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00056877","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=90019604"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=90019604","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670832/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670832/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-10670832/106708322000kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-10670832/106708322000kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-11"}],"displaytype":"detail","filename":"AD-PR-MORI-H-kaken 2002-2p.pdf","filesize":[{"value":"90.9 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"AD-PR-MORI-H-kaken 2002-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/50568/files/AD-PR-MORI-H-kaken 2002-2p.pdf"},"version_id":"bc7efece-4c54-493a-98b8-4a2e5049f476"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"アルツハイマー病における神経移植・薬物効果の核医学的評価に関する基礎的研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"アルツハイマー病における神経移植・薬物効果の核医学的評価に関する基礎的研究"},{"subitem_title":"Fundamental Study on Assessment of Neurotransplantation and Drug Treatment in Alzheimers Disease Using Nuclear Medicine Imaging","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2833"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-04-11"},"publish_date":"2022-04-11","publish_status":"0","recid":"50568","relation_version_is_last":true,"title":["アルツハイマー病における神経移植・薬物効果の核医学的評価に関する基礎的研究"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T13:19:08.467116+00:00"}