{"created":"2023-07-27T06:54:58.647321+00:00","id":50583,"links":{},"metadata":{"_buckets":{"deposit":"ac4251da-4283-448d-a63c-55388661e555"},"_deposit":{"created_by":18,"id":"50583","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"50583"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00050583","sets":["2812:2813:2830"]},"author_link":["20336","71203"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-04-18","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"2002-2003","bibliographic_titles":[{"bibliographic_title":"平成15(2003)年度 科学研究費補助金 基盤研究(C) 研究成果報告書概要"},{"bibliographic_title":"2003 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"昨年度の研究に加え、さらに健常成人の1集団(33名、20-29歳)を対象に、4ヶ月間隔で合計3回に渡りディフィシル菌(Clostridium difficile)の保有状況を検索した。分離菌株をPCRリボタイピング、パルスフィールドゲル電気泳動(PFGE)法を用いて解析することにより、保有状況の特性を検討し、さらにディフィシル菌陽性者と陰性者の腸内細菌叢を比較した。1.ディフィシル菌の保有状況:被験者延べ99人中9人(9.1%)においてディアイシル菌の保有が認められた。各検討時期の保有率は3回共9.1%であり、昨年度の2集団とほぼ同じ保有率であった。保有者は4名であり、2名は3回共陽性、1名は2回陽性、1名は1回陽性を示した。2.ディフィシル菌保有状況の特性:4名から分離した9菌株について遺伝学的解析を行ったところ、4名中3名からの分離菌株が同一の遺伝子タイプを示したが、複数回分離された被験者において、分離された菌株の遺伝子タイプは変化していた。3.腸内細菌叢の比較:昨年度の集団の被験者も含めて、ディフィシル菌の陽性者(12名)、陰性者(12名)を対象に腸内細菌叢の比較を行ったところ、ディフィシル菌陽性者は陰性者に比べて糞便中のEnterococcus属の菌数が有意に多かった。以上の結果は健常成人におけるディフィシル菌の消化管保有は多くの場合一過性であるが、恒常的保有者も存在すること、ディフィシル菌の存在とEnterococcus属の存在との間には相互作用が存在する可能性を示唆している。","subitem_description_type":"Abstract"},{"subitem_description":"In addition of the last year, another one group of healthy individuals(n=33,age range,20-29) was examined for colonization of Clostridium difficile a total of 3 times at intervals of 3 months. Isolates were analyzed by PCR ribotyping and pulsed-field gel electrophoresis(PFGE) to investigate the colonization status of C.difficile. Moreover, intestinal microflora of C.difficile-positive and -negative subjects was compared. 1.Colonization of C.difficile : C.difficile was isolated from nine(9.1%) of 99 samples. Colonization rates were 9.1% at all three examinations and were similar to those in previous study. Among the four positive subjects, the number of subjects from whom C.difficile was isolated once, twice, three times was two, one and one, respectively. 2.Colonization status of C.difficile : All nine isolates form four specimens were analyzed by PCR ribotyping and PFGE. C.difficile of the same PCR ribotype/PFGE type of were isolated from three subjects. The PCR ribotype/PFGE type of the isolated from a subject at the another examination was changing. 3.Comparison of intestinal micro flora : Composition of feacal micro flora was compared in C.difficile-positive(n=12) and -negative(n=12) subjects. C.difficile-positive subjects were significantly more densely colonized by enterococci than C.difficile-negative subjects. These results suggest that colonization of healthy individuals by C.difficile・is transient in many cases, however, indicates that there are healthy individuals who were continuously colonized by C.difficile. Moreover, these data suggest that dense colonization of the intestine by enterococci may be associated with C.difficile colonization.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:14570233, 研究期間(年度):2002-2003","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「ディフィシル菌の感染様式の解明」課題番号14570233\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-14570233/145702332003kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学大学院医学系研究科","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00056892","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=90019620"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=90019620","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14570233/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14570233/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-14570233/145702332003kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-14570233/145702332003kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-25"}],"displaytype":"detail","filename":"ME-PR-NAKAMURA-S-kaken 2005-2p.pdf","filesize":[{"value":"96.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-NAKAMURA-S-kaken 2005-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/50583/files/ME-PR-NAKAMURA-S-kaken 2005-2p.pdf"},"version_id":"41d68d0f-482b-4298-b2a4-8a1d71fc55c1"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ディフィシル菌の感染様式の解明","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ディフィシル菌の感染様式の解明"},{"subitem_title":"Analysis of carriage and transmission of Clostridium difficile","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2830"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-04-25"},"publish_date":"2022-04-25","publish_status":"0","recid":"50583","relation_version_is_last":true,"title":["ディフィシル菌の感染様式の解明"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T13:09:01.750160+00:00"}