{"created":"2023-07-27T06:54:58.873500+00:00","id":50588,"links":{},"metadata":{"_buckets":{"deposit":"28f9d111-35f6-4c94-8eaa-c64b1dbc9c7d"},"_deposit":{"created_by":18,"id":"50588","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"50588"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00050588","sets":["2812:2813:2829"]},"author_link":["91578","22292"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-12-12","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"2003-2004","bibliographic_titles":[{"bibliographic_title":"平成16(2004)年度 科学研究費補助金 基盤研究(C) 研究成果報告書概要"},{"bibliographic_title":"2004 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"今回の研究において,ヒト嗅粘膜を採取し,免疫組織化学的な検討を行うとともに,動物嗅細胞を用いた,培養実験系の確立を目指した準備を行った。\nヒトの嗅粘膜は鼻腔の深部の非常に狭い部位に存在し,直上は薄い筋板を経て頭蓋内であるため,票補運の採取は決して容易ではない。数例の嗅覚障害患者から同意を得て嗅粘膜を採取し,パラフィン包埋標本を作製し,組織学的検討を行っている。その結果,摘出標本に嗅細胞が存在するもの,嗅細胞が存在しないもの,上皮が採取時に脱落して評価ができなかったものに分かれた。嗅細胞が存在するものについては免疫組織学的検討を行い,神経成分の存在を確認できたが,本研究のテーマである分化に関与する因子については有意な結果が出なかった。嗅細胞が存在しない上皮については,嗅覚障害により嗅細胞が変性脱落したものか,採取部位が嗅上皮でなかったのかという問題が含まれている。採取時に上皮が脱落する問題も含め,より安全でなおかつ確実な組織採取を行うために,これまで使用してきた中野式生検鉗子を改良した,新しい鉗子を製作し嗅粘膜の採取を試みたが,十分な嗅粘膜を安定して採取することは困難であった。\n同時に動物を用いた嗅粘膜の組織培養実験も行ったが,残念ながら培養系の確立までにはいたらなかった。","subitem_description_type":"Abstract"},{"subitem_description":"The purpose of this research was to prove the expression of some factors related with differentiation to the mature olfactory neuron in human olfactory mucosa. The olfactory mucosa removed by a special forceps under the patient's agreement were divided three types, existing olfactory neural cells, existing only respiratory column cells and missing any epithelial cells by the mechanical artifacts. Some neural markers were observed-sparsely in the olfactory epithelium by immunohistochemical study. However, any markers related with neural differentiation were not found in the human olfactory epithelium and cultured tissue in this experiment system. It is seemed that more ingenuity is needed at the course of removal, fixation and tissue culture of olfactory epithelium.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:15591801, 研究期間(年度):2003-2004","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「嗅細胞への分化に関与する因子のヒト嗅上皮での発現と培養嗅細胞に及ぼす影響」課題番号15591801\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15591801/155918012004kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学大学院医学系研究科","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00056897","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=20229909"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=20229909","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15591801/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15591801/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15591801/155918012004kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15591801/155918012004kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-25"}],"displaytype":"detail","filename":"ME-PR-MIWA-T-kaken 2007-2p.pdf","filesize":[{"value":"116.8 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-MIWA-T-kaken 2007-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/50588/files/ME-PR-MIWA-T-kaken 2007-2p.pdf"},"version_id":"ab9c3a37-c7dc-42f1-8515-c712774ebbbd"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"嗅細胞への分化に関与する因子のヒト嗅上皮での発現と培養嗅細胞に及ぼす影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"嗅細胞への分化に関与する因子のヒト嗅上皮での発現と培養嗅細胞に及ぼす影響"},{"subitem_title":"Study of the expressions of differenciation factors to olfactory neuron in the olfactory epithelium of human and the other animal","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2829"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-04-25"},"publish_date":"2022-04-25","publish_status":"0","recid":"50588","relation_version_is_last":true,"title":["嗅細胞への分化に関与する因子のヒト嗅上皮での発現と培養嗅細胞に及ぼす影響"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T13:08:54.817380+00:00"}